Culture media Flashcards
1
Q
approaches for cultivation of microorganisms
A
- culture media → petri dish
- cell line cultures (primary, diploid, tumor or immprtalized) → mostly used
- embryonated chicken eggs → intracellular parasites
- laboratory animals
2
Q
general requirements for culture media
A
- agar → makes medium solid
- sterile → filtration/ sterilisation
- isotonic
- neutral pH → some exceptions in acidic or alkaline
- translucent
3
Q
types of culture media
A
- non-selective
- selective
- differential-diagnostic
- specialized
- chemically defined
- transport media
- storage media
4
Q
non-selective
A
support growth without fastidious growth requirements
5
Q
selective
A
- e.g. brilliant green bile agar
- recovery of specific microorganisms
- present in mixture of other m.o.
- media with inhibitors that suppress the growth of unwanted microorganisms
6
Q
differential-diagnostic
A
- made from selective media by adding specif. ingredients
- allow identification of a m.o. in a mixture
- add. of lactose + pH indicator to detect lactose fermenting bacteria
7
Q
specialized
A
- detection of spec. microorganisms that may be fastidious or typ. present in large mixes of m.o.`s
- vitamins
8
Q
chemically defined media
A
- cultivation of mammalian cells
- liquid
- each ingredient is known
- essential AAs and microelements
- cell line cultures
9
Q
transport media
A
- preserve specimen and minimize bact. overgrowth from time of collection to time its received at laboratory to be processed
- 24 hours but asap
- minimum ingredients/ nutrients
10
Q
storage media
A
- used for storing m.os for long time (years)
- low temps (-80/-120c) or liquid nitrogen
- glycerol + milk? → cryoprotectors for the membranes of chrystals
