CP07 - Respiration Flashcards
State the apparatus used to measure the rate of respiration of a small organism.
respirometer
Why is soda lime added to the respirometer?
To absorb carbon dioxide in the respirometer so that the only change in volume results from change in oxygen volume
How does the respirometer measure the rate of respiration?
The change in position of a drop of dye in the capillary tube indicates the volume of oxygen consumed for respiration. The rate of oxygen consumption is proportional to the rate of respiration.
State the controlled variables of this practical
Temperature
Mass of soda lime
Time
How is the rate of respiration calculated?
Rate of respiration = volume of oxygen consumed / mass of organism cm3 g-1
State a source of error in this practical
- If using animals, stress in captivity may affect the rate of photosynthesis.
- The apparatus may not be airtight which may affect the movement of the dye.
How is the volume of oxygen consumed measured?
By reading off the markings of the capillary tube, or measuring the distance moved by the dye and find volume using πr2.
How can the reliability of this practical be improved?
By repeating the practical at least 3 times to find the mean volume of oxygen consumed and calculate a mean rate.