Corynebacterium, Listeria, and Bacillus Flashcards

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1
Q

Is Corynebacterium diphtheriae Gram positive or negative, environmental conditions and shape?

A
  • Gram Positive
  • strict Areobe (requires oxygen)
  • Pleomorphic (club shaped)
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2
Q

How can you identify C. Diphtheriae?

A
  • grows on Loeffler’s Medium
  • Stain for polyphoshate granules (stain pink)
  • cell stains blue
  • Metachromatic

Growth on Tellurite agar

  • reduction by bacteria
  • tellurium precipitation
  • Black Colonies

Schick skin test

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3
Q

What are the symptoms associated with C. Diphtheriae?

A
  • Infection of the upper respiratory tract (pharynx,larynx,nose,skin)
  • pseudomembrane
  • choking
  • bacteria do not spread systematically
  • mucosal lesions marked with greyish patches with surrounding inflammation
  • Bull Neck (characteristic feture)
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4
Q

Does C. Diphtheriae produce a Toxins?

A

Yes, C. Diphtheriae does produce an AB exotoxin in which the B subunit binds to the host cell and the A subunit inhibits protein synthesis. A characteristic of the toxin is that when ADP-ribose mou=iety (NADH) attaches it is able inhibit ELII.

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5
Q

If so what is a characteristic feature of the C. Diphtheriae toxin?

A

encoded by a bacteriophage tax gene

  • not produced in the presence of iron
  • iron-represor complex forms
  • inhibits expression of the tax gene
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6
Q

What treatment is given to patients who acquire C. Diphtheria?

A

Patients must be treated with an antibiotic and an anti-toxin.
Can be given penicillin or erythromycin and the diphtheria anti-toxin . After infection is eliminated patient must be given an immunization with the toxoid in order to develop immunity.

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7
Q

Prevention of C. Diphtheria includes?

A
  • DPT: Toxoid (+pertussis and Tetanus)

- Found on Normal Flora

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8
Q

What are the Characteristics of Listeria monocytogenes?

A
  • Gram positive (rod shapes smaller than E. colli)
  • Motile (dishtiguishable from Corynebacterium)
  • Catalase-positive (unlike streptococcus)
  • grows in 1-45ºC
    Referidgerator temp.
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9
Q

What are the clinical manifestations and of L. monocytogenes?

A
  • Meningitis (swelling of membranes surrounding the brain)
  • Encephalitis (swelling of the actual brain)
  • Bacteremic infection in pregnant women can cause abortion,stillbirth,or premature birth
  • Neonatal meningitis,bacteremia, or both which can occur though transplacental infection or vaginal delivery.
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10
Q

What are the most important epidemiological facts are associated with L. monocytogenes?

A
  • Foodborne transmission and intrauterine transmission
  • Intestinal colonization of the human reservoir
  • periodic outbreaks associated with dairy products.
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11
Q

What abilities of specific abilities allows for pathogenesis of L. monocytogenes?

A

1) Penetrate host cells of the epithelial lining
2) produces lysteriolysin
3) produces phospholipases

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12
Q

What are the 5 steps associated with the colonization of cultured cells and intracellular spread of L.monocytogenes?

A
  1. Phagocytosis by a macrophage or invasion of non- phagocytic cells mediated by L. monocytogenes membrane protein “internalin.”
  2. Escape from membrane-bound vacuole into host cell cytosol mediated by LLO.
  3. Multiplication of the bacterium in the host cell.
  4. Movement through the host cell cytosol mediated
    by actin polymerization.
  5. Penetration of neighboring host cell membranes.
    Entry into cytosol possibly mediated by
    phospholipase.
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13
Q

When is someone Diagnosed with L. monocytogenes

A

-monocytes seen in the peripheral blood and spinal fluid.
• Hemolysin is important marker, but not definitive.
• Characteristic tumbling motility at 25C.
• Can multiply at low temperatures (enriched
in contaminated food in refrigerator).

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14
Q

What is the treatment given to patients diagnosed with L. monocytogenes?

A
  • Penicillin G

- Ampicillin

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15
Q

Which populations are susceptible to L. Monocytogenes?

A

Increased frequency of listeriosis in people with compromised cellular immune function due to disease (AIDS), immunosuppressive therapy, age, or pregnancy.

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16
Q

What is the morphology and characteristics associated with B. anthracis?

A
  • Gram positive
  • spore-forming
  • encapsulates in 5%CO2
17
Q

What are the routes of infection for B. anthracis?

A
  • inoculation
  • ingestion
  • inhilation
18
Q

What is the natural host for B. anthracis?

A
  • Cattle, Sheep, horses, hogs, and goats

- Humans are incidental hosts

19
Q

What are the 3 forms of anthrax?

A
  • Cutaneous (enter broken skin)
  • Pulmonary
  • Gastrointestinal (not seen in US)
20
Q

What would an cutaneous infection of anthrax look like?

A
  • vesicular papule at the site of infection
  • blue-black edema
  • rupturing of the papule will reveal a black eschar
21
Q

How would inhalation of anthrax infect the lungs?

A
  • spore inhalation and phagocytosis
  • germination,replication and infection hilar lymph nodes
  • hemorrragic necrosis of lymph nodes
  • fever, myalgia, malaise, and non-productive cough
  • respiratory failure 1-2 days later
22
Q

What are the virulence factors associated with B. anthrax

A

Capsule (plasma coded)

  • antiphagocytic
  • plasma encoded
  • antibodies to the capsule are not protective against the disease

Exotoxins (plasma encoded)
protective antigen (PA),plus
-edema factor (EF)=edema toxin
-lethal factor (LF)=lethal toxin

23
Q

How would you treat an individual who has acquired anthrax ?

A
Treatment
Ciprofloxacin (or doxycycline)
with one or more of the following:
•  rifampin
•  vancomycin
•  penicillin
•  imipenem
•  clindamycin
•  clarithromycin
24
Q

How would you prevent from getting anthrax

A
Prevention
•  antibody to the toxin complex is neutralizing and protective
•  two vaccines
-  immunize cattle and other
herbivores
- immunize humans at risk