Core Practical Vitamin C

Question 1

What is the method used to test for Vitamin C detection?

Answer 1

Titration

Question 2

What is titration?

Answer 2

it is the determining quantity of substance present by gradually adding vitamin C solution to the DCPIP

Question 3

what equipements are needed in Vitamin C investigation?

Answer 3

-Vitamin C solutions
-1% DCPIP solution
-Distilled water
-Range of fruit juices
-Measuring cylinder
-Pipette
-Stop watch
-Test tubes

Question 4

what is the method for Vitamin C investigation?

Answer 4

-Get 6 concentration of unknown concentration of Vitamin C content
-use measuring cylinder to get 1cm3 of DCPIP into test tube
-add one of the Vitamin c concentration drop by drop to DCPIP gradually (use pipette)
-Shake the test tube for same set period of time for all concentrations using stopwatch. (Control variable)
-when solution= colorless record volume Vitamin C solution added
repeat step 2-5 to get better average
repeat step 2-6 for all concentrations.

Question 5

what is the control variable for Vitamin C investigation?

Answer 5

Length of time that the test tube if shaken for

Question 6

What colour is DCPIP?

Answer 6

Blue dye

Question 7

What colour is DCPIP in presence of Vitamin C?

Answer 7

colourless

Question 8

what precautions should be taken?

Answer 8

avoid DCPIP contact with skin and wear glasses due to DCPIP being irritant.

Question 9

what should be the overall results of this experiment?

Answer 9

volume of vitamin C solution required to decolourise DCPIP should decrease as the concentration of the vitamin C solution increases

Question 10

what is the independent variable ?

Answer 10

is the concentration of vitamin C

Question 11

what is the dependent variable ?

Answer 11

what we measure DCPIP