Control of gene expression Flashcards

1
Q

A multicellular organism has different types of cells that form what

A

Tissues and organs

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2
Q

Do all cells have the same DNA?

A

Yes

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3
Q

Different cell types produce different ________

A

Proteins

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4
Q

Differentiation is the process by which the cells take on characteristic physiological and morphological traits that are necessary for what cell state

A

Terminal cell state

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5
Q

Differentiation is the result of what

A

Regulation gene expression

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6
Q

General strategies relate to controlling transcription, translation, and the stability of what

A

Activity of mRNA and proteins in a cell

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7
Q

What is a common way to regulate expression

A

To limit the amount of transcription that occurs

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8
Q

What is the general strategy used to regulation gene expression

A

Use DNA-binding proteins to affect how well RNA polymerase binds a promoter

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9
Q

What are operons

A

groups of genes under the control of a single promoter

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10
Q

One promoter is found where in an operon

A

the beginning

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11
Q

One large piece of mRNA is transcribed rather then what

A

and individual RNA molecule

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12
Q

Multiple proteins are produced from where

A

The large transcript

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13
Q

Operons can be used as what

A

How protein activation and inactivation regulate gene expression

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14
Q

Proteins that inhibits transcription are called

A

Repressor proteins

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15
Q

What are activator proteins

A

regulatory proteins that increase transcription by enhancing RNA polymerase binding at the promoter

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16
Q

what are the main functions of trp operons

A

-encodes enzymes to produce tryptophan
-Trpr decreases transcription when it is active

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17
Q

the activity of trp depends on what

A

the amount of tryptophan present in the cell

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18
Q

What does TrpR do

A

Binds an operator site near the trp promoter

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19
Q

TrpR is allosteric with binding sites for what

A

DNA and tryptophan

20
Q

Tryptophan binds TrpR and opens the DNA binding site to activate TrpR which then binds what

21
Q

If there is no tryptophan then what happens to the TrpR

A

it is inactive

22
Q

The lac operon has two DNA binding sites for two different what

A

Regulatory proteins

23
Q

What are the two regulatory proteins

A

Lacl - lac repressor
CAP - catabolite activator protein

24
Q

Both proteins are what

A

Allosteric

25
Lacl can bind DNA and what
Allolactose
26
CAP can bind what
DNA and cAMP
27
lac operon encodes proteins to convert lactose to what
glucose
28
Lacl binds DNA when what is absent
Allolactose
29
CAP binds DNA when what is present
cAMP
30
Eukaryotic cells also use regulatory proteins/transcription factors to control what
Gene expression
31
Binding regions may be far from what
Promoters
32
Transcription factors bind near promoters along with mediators and what
chromosome remodeling proteins
33
Interaction of regulatory proteins with TF's modulates what
RNA polymerase activity
34
Eukaryotes use chromosome structure to regulate what
expression
35
Is compacted DNA easily transcribed?
No
36
DNA can be relaxed to allow what
transcription in those regions and repackaged to decrease transcription
37
Lysine =
NH3+
38
Acetylation =
CH3COO-
39
Are individual proteins alone enough to start transcription
No it may not be
40
What is needed in addition to individual proteins to start transcription
Binding of additional transcription
41
Activity of these proteins may be regulated by compounds such as
Cortisol
42
Many cellular pathways can lead to the activation of what
a transcription factor
43
Bases in single stranded RNA base pair with other bases in the strand and lead to
secondary structure in RNA
44
RNA 2 degree structure can attract what
translation repressors
45
Mirco RNAs base pair with mRNA, which causes what
Decreased stability and translation
46
Small interfering RNA's base pair with viral RNa to do what
Inhibit viral gene expression in host cells
47
Long noncoding RNA's do what
Fold into shapes that tether proteins together to perform in a process