Contributions of ... Flashcards
Koch
Koch’s pastulates
Lister
applied germ theory to medical procedure. treated surgical wounds with carbolic acid.
Semmelwels
wash hands found women dying in hospital while giving birth
Linnaeus
binomial name genus and species italicized and underlined
Pasteur
pasteurization - heating enough to kill microbes
Redi
maggots in purifying meat; spontaneous generation
Jenner
vaccine for smallpox
What is spontaneous generation?
living things arise from non-living things
What are examples of some carbohydrates?
sucrose, lactose, maltose, sucrose, glucose,
What is TEM?
Transmission Electron Microscopy: microscopy technique which beam of electrons is transmitted through a specimen to form an image.
What is SEM?
Scanning Electron Microscopy:
What dyes are used for... acid fast: endospore: capsule: gram: negative: flagella: simple:
- carbol fuchsin, acid alcohol, methylene blue
- Malachite green
- congo red, maneavels
- methylene blue, iodine, decolorizer, safranin counterstain
- nigrosin or congo red
- alcoholic dye or silver nitrate
- methylene blue
What is the flagella structure?
helically shaped structures containing protein flagellin.
Protoplast vs. Spheroplast
protoplast refers to spherical shape by Gram +
Spheroplast is spherical shape by Gram -
Gram stain steps:
- Inoculate organism and heat fix.
- Primary stain: methylene blue for 1 minute
- Rinse with distilled water
- Mordant dye, iodine, 1 minute
- Rinse with distilled water.
- decolorize
- rinse with distilled water.
- counterstain with safranin 1 minute
- rinse with distilled water.