Contributions of ... Flashcards

1
Q

Koch

A

Koch’s pastulates

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2
Q

Lister

A

applied germ theory to medical procedure. treated surgical wounds with carbolic acid.

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3
Q

Semmelwels

A

wash hands found women dying in hospital while giving birth

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4
Q

Linnaeus

A

binomial name genus and species italicized and underlined

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5
Q

Pasteur

A

pasteurization - heating enough to kill microbes

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6
Q

Redi

A

maggots in purifying meat; spontaneous generation

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7
Q

Jenner

A

vaccine for smallpox

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8
Q

What is spontaneous generation?

A

living things arise from non-living things

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9
Q

What are examples of some carbohydrates?

A

sucrose, lactose, maltose, sucrose, glucose,

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10
Q

What is TEM?

A

Transmission Electron Microscopy: microscopy technique which beam of electrons is transmitted through a specimen to form an image.

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11
Q

What is SEM?

A

Scanning Electron Microscopy:

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12
Q
What dyes are used for...
acid fast:
endospore:
capsule:
gram:
negative:
flagella:
simple:
A
  • carbol fuchsin, acid alcohol, methylene blue
  • Malachite green
  • congo red, maneavels
  • methylene blue, iodine, decolorizer, safranin counterstain
  • nigrosin or congo red
  • alcoholic dye or silver nitrate
  • methylene blue
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13
Q

What is the flagella structure?

A

helically shaped structures containing protein flagellin.

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14
Q

Protoplast vs. Spheroplast

A

protoplast refers to spherical shape by Gram +

Spheroplast is spherical shape by Gram -

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15
Q

Gram stain steps:

A
  1. Inoculate organism and heat fix.
  2. Primary stain: methylene blue for 1 minute
  3. Rinse with distilled water
  4. Mordant dye, iodine, 1 minute
  5. Rinse with distilled water.
  6. decolorize
  7. rinse with distilled water.
  8. counterstain with safranin 1 minute
  9. rinse with distilled water.
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16
Q

What are the methods of phosphorylation used to generate ATP?

A

oxidative phosphorylation in aerobic and anaerobic respiration

17
Q

What are the end products of glycolysis, lactic acid, fermentation, alcohol fermentation, and aerobic respiration?

A

Glycolysis: 2 pyruvate, 2 ATP, 2 NADH
Lactic Acid: CO2 and lactic and acetic acids
Fermentation: alcohol, carbon dioxide, ehtyl alcohol
aerobic respiration: ATP

18
Q

What is a bacteriophage?

A

virus that infects bacteria

19
Q

What is a prion?

A

protein that can trigger normal proteins in the brain to fold abnormally.

20
Q

What are the classes of temperature dependent organisms?

A
Pychrophiles range from 0-15
psychrotrophs range 20-45
Mesophiles- moderate temps
Thermophiles- 
Extreme Thermophiles