Connective Tissue DLA

Question 1

Permanen residents of CT

Answer 1

Fibroblasts
Adipose cells
Macrophages/monocytes
Mast cells
Mesenchymal stem cells

Question 2

Transient cell in CT

Answer 2

Lymphocytes
Plasma cells
Eosinophils
Basophils
Neutrophils

Question 3

The germ layer that develops CT

Answer 3

Mesoderm

Question 4

What does fibroblast synthesize

Answer 4

Fibers and ground substance

Question 5

Forms of fibroblast

Answer 5

Spindle-shaped
Active: fibroblasts
Inactive: fibrocytes

Question 6

Function of myofibroblasts

Answer 6

Wound healing

Presence of contractile filaments

Question 7

White fat

Answer 7

Unilocular adipocyte
Signet ring cell
Large fat inclusion, organelles and nucleus pushed to periphery
TAG
Rich blood supply

Question 8

Brown fat

Answer 8

Multilocular, many lipid droplets
Central nucleus
Rich in mitochondria
Highly vascular
Rich in innervation
Lipochrome pigments

Heat production
Location: body neck, abdomen of neonates

Question 9

Macrophages

Answer 9

Derived from monocytes, migrate to CT, differentiate to macrophages

Irregular cell membrane,cytoplasmic extensions = pseudopodia

Produce cytokines, phagocytic

Antigen presenting cells, multinuclear giant cells

Question 10

Name of macrophages in liver, brain, and bone

Answer 10

Kupffer cells
Microglia
Osteoclasts

Question 11

Mast cells

Answer 11

Originate in the bone marrow from precursor cells lacking cytoplasmic granules

Migrate to CT or lamina propria of mucosa, they proliferate and accumulate cytoplasmic granules

Same precursor as basophils

Question 12

Mast cell stain

Answer 12

Metachromasia: mast cell appear purple red instead of blue after stained by metachromatic dye like toluidine blue

Due to change in the electronic structure of the dye molecule after binding to the granular

PAS positive: glycoprotein nature

Question 13

Lymphocytes

Answer 13

Small spherical cell, scant cytoplasm

Condensed, basophilic nucleus

Immune cells: B and T cells

Question 14

Plasma cells

Answer 14

Differentiation of b lymphocytes

Secretes single class of immunoglobulin = glycoproteins

Basophilic cytoplasm (rER)
Clockface nucleus, peculiar distribution of chromatin

Negative Golgi: slightly acidophilic area close the nucleus

Question 15

Eosinophils

Answer 15

Eosinophilic = red granules in the cytoplasm

Condensed, bilobed nucleus

Phagocytosis of antibody-antigen complexes

Kill parasitic worms

Question 16

Collagen is _____philic

Answer 16

Eosinphilic

Question 17

Types of collagen for reticular fibers

Answer 17

type III collagen

Question 18

Staining of reticular fiber

Answer 18

Argyrophilic = silver staining

PAS positive: due to carbohydrate

Question 19

Shape of reticular fiber

Answer 19

Short, thin, branching

First collagen synthesized during wound healing

Question 20

3 stages of elastic fiber

Answer 20

Oxytalan
Elaunin
Elastic

Question 21

Components of elastic fiber

Answer 21

Fibrillin - glycoprotein

Elastic - protein

Question 22

Components of elastin

Answer 22

Glycine, proline, desmosine, isodesmosine

Question 23

Stain for elastic fiber

Answer 23

Orcein, rescorcin, Verhoeff’s

Question 24

GAG of ground substance

Answer 24

Hyaluronic acid
Dermatan sulfate
Chondroitin sulfate
Keratan sulfate
Heparan sulfate

Question 25

Glycoproteins of ground substance

Answer 25

Adhesin moledules:
Fibronectin
Laminin
Chondronectin

Integrins - cell surface receptor

Question 26

Proteoglycans of ground substance

Answer 26

Aggreca
Versican
Decorin
Syndecan