Connective Tissue DLA Flashcards
Permanen residents of CT
Fibroblasts Adipose cells Macrophages/monocytes Mast cells Mesenchymal stem cells
Transient cell in CT
Lymphocytes Plasma cells Eosinophils Basophils Neutrophils
The germ layer that develops CT
Mesoderm
What does fibroblast synthesize
Fibers and ground substance
Forms of fibroblast
Spindle-shaped
Active: fibroblasts
Inactive: fibrocytes
Function of myofibroblasts
Wound healing
Presence of contractile filaments
White fat
Unilocular adipocyte Signet ring cell Large fat inclusion, organelles and nucleus pushed to periphery TAG Rich blood supply
Brown fat
Multilocular, many lipid droplets Central nucleus Rich in mitochondria Highly vascular Rich in innervation Lipochrome pigments
Heat production
Location: body neck, abdomen of neonates
Macrophages
Derived from monocytes, migrate to CT, differentiate to macrophages
Irregular cell membrane,cytoplasmic extensions = pseudopodia
Produce cytokines, phagocytic
Antigen presenting cells, multinuclear giant cells
Name of macrophages in liver, brain, and bone
Kupffer cells
Microglia
Osteoclasts
Mast cells
Originate in the bone marrow from precursor cells lacking cytoplasmic granules
Migrate to CT or lamina propria of mucosa, they proliferate and accumulate cytoplasmic granules
Same precursor as basophils
Mast cell stain
Metachromasia: mast cell appear purple red instead of blue after stained by metachromatic dye like toluidine blue
Due to change in the electronic structure of the dye molecule after binding to the granular
PAS positive: glycoprotein nature
Lymphocytes
Small spherical cell, scant cytoplasm
Condensed, basophilic nucleus
Immune cells: B and T cells
Plasma cells
Differentiation of b lymphocytes
Secretes single class of immunoglobulin = glycoproteins
Basophilic cytoplasm (rER) Clockface nucleus, peculiar distribution of chromatin
Negative Golgi: slightly acidophilic area close the nucleus
Eosinophils
Eosinophilic = red granules in the cytoplasm
Condensed, bilobed nucleus
Phagocytosis of antibody-antigen complexes
Kill parasitic worms
Collagen is _____philic
Eosinphilic
Types of collagen for reticular fibers
type III collagen
Staining of reticular fiber
Argyrophilic = silver staining
PAS positive: due to carbohydrate
Shape of reticular fiber
Short, thin, branching
First collagen synthesized during wound healing
3 stages of elastic fiber
Oxytalan
Elaunin
Elastic
Components of elastic fiber
Fibrillin - glycoprotein
Elastic - protein
Components of elastin
Glycine, proline, desmosine, isodesmosine
Stain for elastic fiber
Orcein, rescorcin, Verhoeff’s
GAG of ground substance
Hyaluronic acid Dermatan sulfate Chondroitin sulfate Keratan sulfate Heparan sulfate
Glycoproteins of ground substance
Adhesin moledules:
Fibronectin
Laminin
Chondronectin
Integrins - cell surface receptor
Proteoglycans of ground substance
Aggreca
Versican
Decorin
Syndecan
