computers and specimen handling and processing Flashcards

(35 cards)

1
Q

What are computers used for in healthcare?

A

to manage data (information collected for analysis or computation)
identify and monitor patients
automate analyzers
aid in diagnosis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

it is defined as having basic knowledge about computers and how to use them

A

computer literacy

  • to be considered literate, an individual must be able to:
    Know basic computer terminology
    Understand the computer and its functions
    Perform basic operations using computers
    Be willing to adapt to the challenges computers bring
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the requirements to be considered computer-literate?

A

Know basic computer terminology.
Understand the computer and its functions.
Perform basic operations using computers.
Be willing to adapt to the challenges computers bring.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

it is the specialized application of information technology to develop, maintain, and use computers, systems, and networks to store, retrieve, and send information for optimizing laboratory operations.

A

Laboratory informatics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

It is a customized computer software package designed to record, process, manage and store data from a variety of workflow processes in the laboratory.

A

laboratory information system (LIS)
*major part of the laboratory informatics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What are the functions of a Laboratory Information System (LIS)?

A

records
processes
manages
stores
- data from a variety of workflow in the lab

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the three phases of the laboratory process in specimen handling and processing?

A

Pre-examination (pre-analytical): Prior to testing or analysis

Examination (analytical): During testing or analysis

Post-examination (post-analytical): After testing or analysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In which phase do most laboratory errors occur?

A

preexamination (preanalytical) phase
- which begins when a patient is assessed and a test is ordered

*hence it is critical as the quality of results depends on following correct procedures, and errors in this phase can significantly impact test outcomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Why is the preexamination phase critical in specimen handling and processing?

A

it is critical because the quality of results depends on following correct procedures, and errors in this phase can significantly impact test outcomes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

possible causes that errors that might occur before collection

A

Altitude
Dehydrated patient
Duplicate test orders
Exercise
Inadequate fast
Incomplete requisition
Medications
Patient stress
Pregnancy
Smoking
Strenuous exercise
Treatments (e.g intravenous medications, radioisotopes)
Wrong test ordered

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

possible causes that errors that might occur at time of collection

A

Misidentified patient
Antiseptic not dry
Expired tube
Failure to invert additive tubes properly
Faculty technique
Improper vein selection
Inadequate volume of blood
Inappropriate use of plasma separator tube (PST) or serum separator tube (SST)
Incorrect collection tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

possible causes that errors that might occur during specimen processing

A

Contamination (e.g, dust or glove powder)
Delay in processing or testing
Delay in fluid separation from cells
Evaporation
Failure to centrifuge specimen according to test requirements
Failure to separate fluid from cells
Incomplete centrifugation
Mislabeled aliquot
Multiple centrifugation
Rimming of clots

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Why is it important to handle and transport blood specimens immediately?

A

to prevent delays in separating blood cells from plasma or serum
as delays can cause metabolic changes in the sample

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

it is an automated transportation system that uses a network of long tubes to connect sending and receiving stations in the laboratory to stations in various hospital areas, such as nurses’ stations.

A

Pneumatic Tube System (PTS or P-tube)

*one of the most common means of transporting specimens to the laboratory

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

time limit exceptions of ammonia

A

blood ammonia levels increase rapidly at room temperature
- immediately placed in an ice slurry or a cooling tray after collection
- transported STAT and separated from the cells within 15 minutes of collection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the ideal time frame for performing PT and PTT tests after specimen collection?

A

PT and PTT tests should ideally be performed within 4hours of specimen collection.
- best if all specimens for plasma-based coagulation tests are processed as soon as possible after collection.

17
Q

PT vs PTT

A

PT test measures :
how long it takes for plasma in the blood to clot, helping identify bleeding and clotting disorders.

PTT test measures:
how long it takes for blood to clot and helps identify bleeding problems or diseases

[PT (Prothrombin Time):
time for plasma to clot via extrinsic and common pathways
PTT (Partial Thromboplastin Time):
time for blood to clot via intrinsic and common pathways]

18
Q

How long are glucose specimens drawn in sodium fluoride tubes stable at
- room temp:
- when refrigerated:

A

room temp: 24hrs
when refrigerated: 48hrs (4-8°C)

19
Q

Why should glucose specimens from newborn and pediatric patients be tested as soon as possible?

A

as it is difficult to inhibit glycolysis in newborn and pediatric specimens
- which can affect test accuracy

20
Q

What type of device is typically used to collect glucose specimens from newborn and pediatric patients?

A

microcollection devices are used
- allowing a small amount of blood to be collected

21
Q

How soon must blood smears from EDTA specimens be prepared after collection?

A

within one hour of collection

22
Q

How long are EDTA specimens for CBCs stable at room temperature?

A

analyzed within 6 hours
but are generally stable for 24 hours at room temperature

23
Q

What is the testing time frame for EDTA specimens for ESR determinations?

A

Within 4 hours if left at room temperature
Within 12 hours if refrigerated

24
Q

What does the erythrocyte sedimentation rate (ESR) measure, and what does an elevated ESR indicate?

A

ESR measures the rate at which red blood cells settle at the bottom of a vertical tube over a specified period, usually one hour

An elevated ESR indicates the presence of inflammation in the body

25
How long are EDTA specimens for reticulocyte counts stable?
Up to 6 hours at room temperature. Up to 72 hours if refrigerated
26
Why must plasma preparation tubes (PPTs) for molecular testing be processed as soon as possible?
as RNA substances/materials are extremely unstable - molecular testing, such as hepatitis C RNA tests *if cannot run immediately = stored at 4°C for up to 48 hours if not tested within that time, it must be transferred to an aliquot tube and frozen at -80°C
27
What should be done if an RNA test cannot be run immediately?
The plasma can be stored at 4°C for up to 48 hours. If not tested within that time, it must be transferred to an aliquot tube and frozen at -80°C.
28
How long can plasma for RNA tests be stored at 4°C before it needs to be frozen?
It can be stored for up to 48 hours.
29
What types of specimens are collected for microbiology cultures?
blood urine throat swabs wound samples
30
How long does it typically take to process a microbiology culture?
takes 18 to 24 hours to process - important to transport and process microbiology specimens quickly - to preserve microorganisms in the specimens so they can be properly identified
31
How should urine specimens be handled if they cannot be transported to the lab immediately?
They can be held at room temperature and protected from bright light for up to 2 hours. *prompt transport ensures accurate test results by preventing degradation or changes in the specimen
32
What should be done with urine specimens that need to be held for longer than 2 hours?
should be refrigerated
33
It involves the possibility of exposure to blood borne pathogens (BBP)
specimen processing
34
What do OSHA BBP regulations require for specimen processors?
they must wear PPE
35
What are examples of specimen rejection criteria due to contamination?
1. Contaminated specimen - Urine C&S in an unsterile container 2. Delay in processing - Glucose specimen that was not separated from the cells until four hours after collection 3. Hemolysis - Hemolyzed potassium specimen 4. Inadequate, inaccurate or missing patient ID - Urine specimen that is not labeled 5. Outdated tube 6. Wrong tube 7. QNS (quantity not sufficient)