Clinical Microbiology Laboratory

Question 1

What types of pathogens are microscopy used for?

Answer 1

> bacteria
fungi
viruses
parasites

Question 2

What kind of cells produce monoclonal antibodies (mAb)?

Answer 2

hybridoma cells

Question 3

Do monoclonal Abs recognize a single or multiple epitopes?

Answer 3

Single Epitope

Question 4

What are chimeric mAbs?

Answer 4

Genetically modified mice that produce Ab with a human constant region.

Question 5

What are humanized mAbs?

Answer 5

mAbs that are mostly human, except for mouse antigen-binding.

Question 6

What are fully human antibodies?

Answer 6

mAbs produced from a human gene on a mouse.

Question 7

What is a hybridoma cell?

Answer 7

“immortal” cancerous B cell fused with an antibody-producing normal B cell.

Question 8

What have fluorescently-labeled mAbs replaced the use of for culture confimation?

Answer 8

polyclonal antisera

Question 9

What techniques are typically used in the identification of parasites?

Answer 9

> histological staining of blood
negative staining of other body fluids
immunofluorescence staining

Question 10

List the molecular methods widely used in the lab.

Answer 10

> nucleic acid proves; DNA hybridization
> PCR and RT-PCR
> ribotyping (16S rRNA analysis)
> multilocus sequence typing (MLST)
> genomic fingerprinting
> genomic fingerprinting
> plasmid fingerprinting

Question 11

Stopped on slide 30

Answer 11

In lecture: ribotyping