Clinical Microbiology

Question 1

Aggultination

Answer 1

Direct or Indirect
Direct- Antibody and antigen, clumps

Indirect- with latex beads
Latex coated with antigen - if clumps occur then patient has antibodies
Latex coated with antibodies - if combs occur then patient has the antigen

Question 2

Hemoaggultination

Answer 2

Aggultination of RBCs
Virus’s can do this:
Antibodies can neutralize viruses from clumping blood, so if the patient has serum antibody no blood clumping occurs

Question 3

Complement Fixation

Answer 3

Direct
-Positive: Patient has Ab -> complement binds Ab-Ag complex and no RBC lysis
-Negative: No Ab -> complement lysis blood cell
Indicator: RBC
Indirect Test