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Micro final exam > Clinical Microbiology > Flashcards

Clinical Microbiology Flashcards

1
Q

isolate and identify pathogenic microorganisms from specimens, quickly

A

clinical microbiologists

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2
Q

non-pathogens
general safety standards (PPE as needed)
door and a sink

A

BSL1

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3
Q

human disease, moderate hazard, “indigenous”

A

BSL2

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4
Q

indigenous or exotic, serious or lethal disease by respiratory transmission

A

BSL3

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5
Q

Dangerous & exotic, high risk aerosol transmission

A

BSL4

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6
Q

potentially infected human material collected for testing

A

clinical sample

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7
Q

aseptically
representative of infected site
adequate quantity for testing
appropriate container
before antibiotics given

A

specimen collection for clinical sample

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8
Q

suprapubic aspiration
clean catch
catheter

A

urine collection

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9
Q

fecal samples
sputum

A

other samples expected by patients

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10
Q

swab
needle aspiration
biopsy, surgical removal

A

wound & abscesses collection

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11
Q

use anaerobic transport vials, move quickly

A

anaerobes

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12
Q

direct examination
staining

A

microscopy

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13
Q

direct: detect presence of pathogens
indirect: detect presence of antibodies to pathogen

A

immunofluorescence

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14
Q

says this is the pathogen that is present in the sample

A

direct

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15
Q

antibodies attach to the pathogen saying pathogen might be there or has been there (looking for the antibody)

A

indirect

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16
Q

grow in cell culture (takes awhile for them to grow)
look for cytopathic effects (changes in the cell)

A

viruses (growth)

17
Q

fungal media
colony morphology
- does it look fuzzy

A

fungi (growth)

18
Q

difficult to grow
most are intracellular parasites

A

protozoans (growth)

19
Q

nutrients to support growth of a lot of different microbes

A

rich media

20
Q

inhibiting growth of one thing but letting another grow

A

selective

21
Q

something is growing, changing, or color change

A

differential

22
Q

check for antibodies to pathogen
skin tests

A

immunological tests

23
Q

patient has to have both proteins positive (GP41 & P24) to have ____

A

HIV

24
Q

antigen in the sample shows that the _______ is there

A

pathogen

25
Q

present of the ______ shows the pathogen has been there, is there, or has been vaccinated

A

antibody

26
Q

tests for metabolites and lipids

A

gas-liquid chromatography

27
Q

superseding phenotypic tests
identify metabolites & proteins

A

mass spectrometry

28
Q

measures levels of specific DNA fragments or RNAs
fluorescent probes incorporated during PCR
measure intensity

A

quantitative PCR

29
Q

sample is taken to bioreceptor or electrical interface, transducers, electronic system

A

biosensors

Micro final exam (6 decks)

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