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LABS > CLINICAL CHEMISTRY 2 LAB MIDTERM > Flashcards

CLINICAL CHEMISTRY 2 LAB MIDTERM Flashcards

1
Q

GLUCOSE REFERENCE RANGE

A

3.6-6.0 mmol/L

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2
Q

ALBUMIN REFERENCE RANGE

A

33-45 g/L

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3
Q

UREA REFERENCE RANGE

A

2.8-7.1 mmol/L

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4
Q

SODIUM REFERENCE RANGE

A

135-147 mmol/L

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5
Q

POTASSIUM REFERENCE RANGE

A

3.5-5.1 mmol/L

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6
Q

CHLORIDE REFERENCE RANGE

A

97-106 mmol/L

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7
Q

TCO2 REFERENCE RANGE

A

22-30 mmol/L

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8
Q

CALCIUM, TOTAL REFERENCE RANGE

A

2.10-2.60 mmol/L

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9
Q

CALULATED OSMOLITY FORMULA

A

2(Na) + Glucose + Urea

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10
Q

ANION GAP FORMULA W/O K

A

Na - (Cl+TCO2)

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11
Q

ANION GAP W K FORMULA

A

(Na + K) - (Cl + TCO2)

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12
Q

CORRECTED CALCIUM FORMULA

A

Measured total calcium +( 0.02 [40-albumin g/L])

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13
Q

OSMOLARITY GAP FORMULA

A

Measured osmolity - calculated osmolity, MUST BE WITH +/- 10 OF THE MEASURED

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14
Q

HCO3 CALCULATED

A

ANTILOG (PH-6.10) x (TCO2 x 0.0307)

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15
Q

LDL-C FORMULA

A

TOTAL CHOLESTEROL - HDL CHOLESTEROL - (Triglycerides x 0.46)

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16
Q

NON HDL CHOLESTEROL

A

TOTAL CHOLESTEROL - HDL CHOLESTEROL

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17
Q

What technology is used on the Cobas e 411

A

Electrochemiluminescence

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18
Q

WHAT HAPPENS TO PSA AS YOU AGE?

A

IT INCREASES

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19
Q

Both triglyceride and total cholesterol enzymatic reactions end in color production. What enzyme (in both reactions) creates this end product from peroxide and dye?

A

PEROXIDASE

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20
Q

In the enzymatic measurement of triglycerides, bacterial lipase breaks down triglyceride and water to

A

GLYCEROL AND 3 FATTY ACIDS

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21
Q

What is the wavelength used to detect the end product in the enzymatic measurement of total cholesterol

A

500NM

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22
Q

Using plasma from an under-filled sodium or lithium heparin tube will falsely…….

A

INCREASE RESULTS

23
Q

A corrected calcium result should be calculated when which other analyte is low?

A

ALBUMIN

24
Q

What is present in the blood of a patient suffering from diabetic ketoacidosis that would cause an increased anion gap result?

A

ketone bodies

25
Q

METHOD OF MEASUREMENT OF GLUCOSE ON ISTAT

A

Amperometry

26
Q

METHOD OF MEASUREMENT OF UREA ON ISTAT

A

Potentiometry

27
Q

METHOD OF MEASUREMENT OF HEMATOCRIT ON ISTAT

A

Conductometry

28
Q

VITROS ISE METHOD?

A

Direct ISE Potentiometry System

29
Q

COBAS C 311 ISE METHOD?

A

Indirect ISE Potentiometry System

30
Q

iSTAT ISE METHOD?

A

Direct ISE Potentiometry System

31
Q

TOTAL PROTEIN REFERENCE RANGE

A

60-80 g/L

32
Q

CREATINE REFERENCE RANGE

A

FEMALE: 35-97 umol/L
MALE: 44-106 umol/L

33
Q

TOTAL BILIRUBIN REFERENCE RANGE

A

5-21 umol/L

34
Q

DIRECT BILIRUBIN REFERENCE RANGE

A

<7.0 umol/L

35
Q

LIPASE

A

Catalyzes the hydrolysis of fat, acute pancreatitis

36
Q

LACTATE DEHYDROGENASE

A

Interconversion of lactic and pyruvic acids, coenzyme NAD+, substrate L lactate. Megaloblastic anemia

37
Q

ALKALINE PHOSPHATE

A

Dephosphorylation of compounds,

Hepatobiliary disorders

38
Q

CREATINE KINASE

A

Regeneration of ATP in muscle. Myopathies

39
Q

UNCONJUGATED BILIRUBIN

A

bound to albumin, transport to liver INDIRECR

40
Q

CONJUGATED BILIRUBIN

A

conjugated with glucuronic acid by UDPGT to from conjugated bilirubin to eventually be excreted in urine DIRECT

41
Q

In circulation______ of total bilirubin is unconjugated and______is conjugated bilirubin.

A

80%, 20%

42
Q

ERRONEOUS BILIRUBIN RESULTS

A

Presence of hemolysis, exposed to light

43
Q

What is present in the total bilirubin reaction that is not in the direct bilirubin reaction?

A

SURFACTANT

44
Q

Both total and direct bilirubin mixtures are read using a spectrophotometer set at

A

546 nm

45
Q

Post-hepatic jaundice

A

Increase in conjugated bilirubin

Normal unconjugated bilirubin

46
Q

Hepatic jaundice

A

Increase in unconjugated biirubin

Increase in conjugated bilirubin

47
Q

Pre-hepatic jaundice

A

Increase in unconjugated bilirubin

Normal conjugated bilirubin

48
Q

ALT

A

SPECIFIC FOR LIVER DISEASE

49
Q

GGT

A

IN CHRONIC ALCHOLICS

50
Q

Which liver enzymes are determined by measuring a decrease in absorbance?

A

AST, ALP

51
Q

Total and direct bilirubin are both measured using the ____method

A

diazo

52
Q

Cholestasis

A

Increased total bilirubin

Increased direct bilirubin

Elevated liver enzymes (ALP and GGT)

53
Q

Hemolytic anemia

A

Increased total bilirubin

Normal direct bilirubin

Normal liver enzymes

54
Q

Cirrhosis

A

Increased total bilirubin

Increased direct bilirubin

Elevated liver enzymes (ALT, AST, and LDH)

LABS (8 decks)

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