Clinical Chemistry Flashcards
Random errors
12S, 13S, R4S
1 control value exceeds the mean +/- 2s
12S / Warning rule
1 control value exceeds the mean +/- 3s
13S
1 control value exceeds +2s and ANOTHER value exceeds -2s
R4S
2 CONSECUTIVE control values exceeds the same mean +/- 2s
22S
8 consecutive values fall on 1 SIDE of the mean
8x
Errors that occurs BY CHANCE
Random errors
Errors that are consistent in ONE DIRECTION
Systematic
RANDOM OR SYSTEMATIC:
Pipetting errors, mixing, timing (variation in handling techniques)
Random
RANDOM OR SYSTEMATIC:
Variation in operators
Random
RANDOM OR SYSTEMATIC:
Environmental conditions
Random
RANDOM OR SYSTEMATIC:
Fluctuations in line voltage
Systematic
RANDOM OR SYSTEMATIC:
Technologist interactions
Systematic
RANDOM OR SYSTEMATIC:
Dispensing of reagents
Random
RANDOM OR SYSTEMATIC:
Aging reagents
Systematic
RANDOM OR SYSTEMATIC:
Aging calibrators
Systematic
RANDOM OR SYSTEMATIC:
Calibrator reconstitution
Random
RANDOM OR SYSTEMATIC:
Temperature analyzer
Random
RANDOM OR SYSTEMATIC:
Instability of instrument
Random
RANDOM OR SYSTEMATIC:
Wear and tear of instruments and instrument components
Systematic
ABRUPT change in the mean
Shift
Main cause of shift
Improper calibration of instrument
Drift of values to ONE SIDE of the mean
Trend
Main cause of trend
Deterioration of reagents
Variables: CHAXI
Concentration
Horizontal
Abscissa
X-Axis
Imdependent
Variables: AVOYD
Absorbance
Vertical
Ordinate
Y-Axis
Dependent
F-test
SD and Precision
T-test
Mean and Accuracy
Interlab QC; long-term monitoring; monthly or quarterly
External QC
Intralab QC; short-term monitoring; daily
Internal QC
Solution that uses 1 ANALYTE for CALIBRATION or REFERENCE
Standard
Solution that uses 2 ANALYTES to ENSURE CORRECT RESULTS
Control
Isolates specific or individual wavelength of light
Monochromator
Measures LIGHT TRANSMITTED by a solution to determine the concentration
Spectrophotometer
Determines the amount of LIGHT BLOCKED by a particulate matter in a turbid solution
Turbidimetry
Determines the amount of LIGHT SCATTERED by a particulate matter in a turbid solution
Nephelometry
Measure the amount of LIGHT EMITTED by a molecule AFTER EXCITATION by EMR, over a zero background
Fluorometry
Measures the electrical potential / DIFFERENCES BETWEEN 2 ELECTRODES in terms of voltage at constant current
Potentiometry
Samples flow through a COMMON vessel or pathway
Continuous Flow Analysis
Each sample-reagent mixture is handled SEPARATELY in its own reaction vessel
Discrete Analysis
Multiple tests one sample at a time; most popular and versatile type of automation
Discrete analysis
Uses acceleration and deceleration of ROTOR to transfer the reagents and sample
Centrifugal Analysis
Vertical when not spinning horizontal when spinning
Horizontal Head centrifuge / Swinging bucket centrifuge
Speed of swinging bucket centrifuge
1,650 g
Fixed angle centrifuge with speed of 9000
Angle-head centrifuge
Has continuous ETCHED RINGS on top; exact volume obtained when LAST DROP IS BLOWN OUT
Blowout pipet
The simplest pipet
Pasteur pipet
Relies on PISTON FOR SUCTION creation to draw sample into a DISPOSABLE TIP
Air displacement pipet
Reagent water for preparation of STANDARD SOLUTIONS
Type 1
GENERALLY USED reagent water for chemistry, microbiology, hematology, and immunology; also used for WASHING GLASSWARES
Type 2
Reagent water for urinalysis, parasitology, and histology
Type 3
Bleach inactivates HBV for how many minutes?
10
Bleach inactivates HIV for how many minutes?
2
Diurnal variation: Increase in the MORNING (AM)
🌞*ACIA
ACTC
Cortisol
Iron
Aldosterone
Diurnal variation: Increase in the AFTERNOON (PM)
🌛*GAPT
Growth hormone
ACP
PTH
TSH
Affected by posture; increases from supine to sitting / standing
Albumin
Calcium
Enzymes
*I CLAAAAAMPP
Iron, Catecholamines, CK, LD, ALT, AST, ALP, ACP, Ammonia, Magnesium, Potassium, Phosphate _____ when sample is HEMOLYZED
Increases
If antecubital veins are unsuitable for venipuncture, what are the alternative sites?
Wrist and hand
When BP cuff is used as a tourniquet, it should be inflated at ___ mmHg
60 mmHg
Blood specimens on serum separator gel tubes must be processed within _____
30 minutes
Normal BMI
18.5 - 24.9
BMI of underweight / malnourished
< 18.5
BMI of obese
30 and above
SI unit for length
Meter (m)
SI unit for mass
Kilogram (kg)
SI unit for Themodynamic temperature
Kelvin (K)
SI unit for amount of substance
Mole (mol)
10^6
Mega
10^3
Kilo
10^9
Giga
10^12
Tera
10^-3
Milli
10^-6
Micro
10^-9
Nano
10^-12
Pico
Celsius to Farenheit
F = (C x 1.8) + 32
Farenheit to Celsius
C = (F - 32) x 5/9
Conversion factor of glucose
0.0555
BUN to Urea
2.14
Conversion factor of BUN
0.357
Conversion factor of creatinine
88.4 umol/L
Conversion factor of uric acid
0.0595
Conversion factor of triglycerides
0.0113
Conversion factor of sodium, potassium, chloride
1
Conversion factor of cholesterol
0.026
Conversion factor of TPAG
10 (g/dL) 0.01 (mg/dL)
Conversion factor of bilirubin
17.1 umol/L
Conversion factor of thyroxine
12.9 nmol/L
Effect of high WBC count on blood glucose determination
Decreased
Uses arsenomolybdate reagent
Nelson-Somogyi
Uses phosphomolybdate reagent
Folin-Wu
Results produced by REDUCTION methods for glucose are _____ higher than those of ENZYMATIC methods
5-15 mg/dL
To establish Diabetes Mellitus, symptoms (3P’s) should be accompanied by RBS level of:
200 mg/dL
Beta-cells destruction
Type 1 DM
Insulin resistance
Type 2 DM
Detectable C-peptide levels
Type 1 DM
DM with Autoantibodies
Type 1 DM
Common DM
Type 1 DM
Uses oral agents as medication
Type 2 DM
Uses insulin absolute as medication
Type 1 DM
Glycogen storage disease with G6PD deficiency
Von Gierke
Most common phospholipid found in cell membranes
Lecithin
Apo A1
HDL
Apo B100
LDL, VLDL
Apo B48
Chylomicrons
Reverse cholesterol transport
HDL
Endogenous triglycerides
VLDL
Exogenous triglycerides
Chylomicrons
Least dense
Chylomicrons
Most dense
HDL
Lipoprotein that carries TRIGLYCERIDES
CM, VLDL
Lipoprotein that carries CHOLESTEROL
LDL
Lipoprotein that carries PROTEIN and PHOPHOLIPIDS
HDL
Bad cholesterol
LDL
Good cholesterol
HDL
SPE Migration Pattern of lipoproteins
Chylomicrons (origin)
LDL
VLDL
HDL (anode)
Lipoprotein ultracentrifugation pattern
Chylomicrons (top)
VLDL
LDL
HDL (bottom)
Floating B-lipoprotein
B-VLDL
Sinking Pre-B-lipoprotein
Lp(a)