Clinical Biochemistry

Question 1

Method of separation of serum and plasma

Answer 1

By centrifugation
By high speed rotation according to densities of contents of blood

Question 2

After centriugation

Answer 2

Lower denser layer of blood cells and platelets as they are heavier called pellet layer
Upper lighter layer of plasma called suprantant layer

Question 3

Plasma

Answer 3

Part of blood conating serum and clotting factors
Plasma obtained by centrifugation of blood before clotting occurs

Question 4

Serum

Answer 4

Part of blood that remains once clotting factors like fibrin has been removed
Yellow watery part of blood left after fibrin clot formed in blood has been removed

Question 5

Spectrophotometery

Answer 5

Measurement of the intensity of light at selected wavelength
Used to measure how much solution absorbs light by measuring amount of light passing through the solution

Question 6

Colorimetric kit

Answer 6

Colorimetric reagent
Standard solution
Written protocol to follow
Solution or sample must be colored or can be colored

Question 7

Colorimetric reagents

Answer 7

Have substances that bind o analyze like glucose giving a specific color
Color intensity is directly proportional to concentration of the analyte

Question 8

Compare the sample to a standard
solution

Answer 8

A standard solution has a precisely known concentration
e.g. standard solution of glucose has 100 mg/dl glucose

Question 9

Types of pipette

Answer 9

Fixed and variable
Variable pipette can be adjusted using knob
Should always use smaller pipette according to volume of sample

Question 10

Single or multi channeled pipette

Answer 10

If multiple and same quantity of solution will be added in successive wells use multiple channel pipette

Question 11

Cuvette for colorimeter

Answer 11

Held by fingers from corrugated part

Question 12

Types of cuvette

Answer 12

Optical glass
Up quartz
Ir quartz
Sapphire

Question 13

Calculation of the value of results

Answer 13

Concentration of standard multiplied by value for test divided by value for standard solution so t/sxs

Question 14

What tests can be done by
spectrophotometer

Answer 14

Determination of concentrations of proteins,
glucose, TAG, urea, creatinine, AST, ALT and
many others

Measurements of enzyme activity

Following DNA and RNA extraction to detect
sample purity