citric acid cycle (4) Flashcards
where does the citric acid cycle occur in the cell? oxidative phosphorylation?
both in the mitochondria matrix
about how many ATP does CAC provide? oxidative phosphorlation?
CAC- 2 ATP
ox phos- 32
what membrane of the mitochrondira forms the cristae?
the inner membrane
where are the enzymes for the CAC?
in the fluid of the matrix space
where are the enzymes for oxidative phosphorylation?
embedded in the inner membrane
list the 3 functions of the citric acid cycle
- convert a number of diff fuels to a common mobile fuel (NADH)
- serves as a final meeting place of nearly all oxidizable substrates
- provides intermediates for biosynthesis
list 3 organs that mitochondria are most abundant in
- heart
- kidney
- liver
how does NADH get into the mitochondria?
it does’t! there is a population outside the cell and a population inside the cell and they do not mix so there is no transport machinery for it
what are the 3 enzymes involved in the pyruvate dehydrogenase complex?
- pyruvate dehydrogenase
- dihydrolipoyl transacetylase
- dihyrolipoyl dehydrogenase
what is the pyruvate dehydrogenase complex?
the connection between glycolysis and citric acid cylce
what type of reaction does pyruvate dehydrogenase (E1) do? what prosthetic is associated with it?
a condenstaion/decarboxylation
TPP group
what type of reaction does dihydrolipoyl transacetylase (E2) do? what prosthetic group is associated with it?
oxidative transfer and transacetylation
lipoaminde prosthetic group
what type of reaction does dihydrolipoyl dehydrogenase (E3) do? what prosthetic group is associated with it?
dehydrogenation reaction
FAD prosthetic group
what is the enzyme for the 1st step of the citric acid cycle?
citric synthase
what are the products of the 1st step in the CAC?
citrate and H2O
what type of reaction is the 1st step in the CAC?
a condensation and hydrolysis
what is the enzyme for the 2nd step of the citric acid cycle?
aconitase
what are the products of the 2nd step in the CAC?
cis-aconitase
isocitrate
what type of reaction is the 2nd step in the CAC?
dehydration to make cis-aconitase and a rehydration to make isocitrate
what is the enzyme for the 3rd step of the citric acid cycle? products? type of reaction?
enzyme: isocitrate dehydrogenase
product: alpha-ketogluterate & CO2 & NADH
type of rxn: oxidative decarboxylase
what is the enzyme for the 4th step of the citric acid cycle? products? type of reaction?
enzyme: alpha ketogluterate dehydrogenase
product: succinyl CoA, CO2 and NADH
rxn: oxidative decarboxylation
what is the enzyme for the 5th step of the citric acid cycle? products? type of reaction?
enzyme: succinyl coA synthase
product: succinate & GTP
rxn: substrate level phosphorylation
what is the enzyme for the 6th step of the citric acid cycle? products? type of reaction?
enzyme: succinate
product: fumerate & FADH2
rxn: dehydration
what is the enzyme for the 7th step of the citric acid cycle? products? type of reaction?
enzyme: fumerase
product: malate
rxn: hydration
what is the enzyme for the 8th step of the citric acid cycle? products? type of reaction?
enzyme: malate dehydrogenase
product: oxaloacetate
which step in the CAC demonstrates the common intermediate principal?
the succinyl coA to succinate with the succinyl coA synthase enzyme
