CHROMATOGRAPHY & MASS SPEC

Question 1

what can the spectrophotometer detect

Answer 1

intensity of transmitted light/ electromagnetic radiation

Question 2

what are the 4 major components of a spectrophotometer

Answer 2

light source, monochromator, cuvett, detector

Question 3

what is the detector in a spectrophotometer

Answer 3

phototube

Question 4

what can the mass spectrophotometer detect

Answer 4

intensity of ionic current

Question 5

what are the major components of a mass spec

Answer 5

ion source, mass analyzer, sample injector, and detector

Question 6

what are the 2 types of ion source

Answer 6

soft and hard

Question 7

what are the 2 types of soft ion source

Answer 7

electro spray ionization (ESI) and atmospheric pressure chemical ionization (APCI)

Question 8

what kind of charge and type of sample use ESI

Answer 8

polar and liquid

Question 9

what kind of charge and type of sample use APCI

Answer 9

non polar and gas

Question 10

mass analyzer?

Answer 10

used for the separation of ions in mass spec

Question 11

what are the 3 types of mass analyzers

Answer 11

quadruple, ion trap, and time of flight (TOF)

Question 12

what kind of detector is in a mass spec

Answer 12

electron tube (multiplier)

Question 13

what is the principle measurement of chromatography

Answer 13

separation of compounds through physical interaction

Question 14

what are the 4 major components of chromatography

Answer 14

stationary phase, mobile phase, separation mode, and High-performance liquid chromatography(HPLC)

Question 15

during the separation mode in chromatography what factors deal with separation

Answer 15

absorption, partition, size, ion-exchange

Question 16

what are the 4 parts of the HPLC

Answer 16

pump, injector, column, detector

Question 17

what does the detector detect in the HPLC

Answer 17

detects compound’s mass, ABS, fluorimetry (FI)