chpt 8: microbial growths Flashcards

1
Q

study of modification of gene expression is known as

A

epigenetics

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2
Q

function of hydrogen bonds

A

provide stabilty

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3
Q

which molecule determines the parallel effect

A

sugar

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4
Q

what molecule turns to bring bases close and allows extreme stability

A

sugar

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5
Q

what is semi-conservative replication

A

1 new strand with 1 old/ parental strand

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6
Q

DNA helicase
DNA gyrase
DNA polymerase
RNA polymerase
DNA ligase
are involved in

A

semi-conservative replication

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7
Q

function of DNA helicase

A

unwinds/ unzips the helix

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8
Q

which enzyme is responsible for stabilizing unwound parent DNA

A

DNA gyrase

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9
Q

when is the replication strand formed

A

after the DNA is unwound

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10
Q

two strands of replication fork is

A
  • leading strand
  • lagging strand
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11
Q

which direction does the leading strand run from

A

5’-3’

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12
Q

function of DNA polymerase

A
  • proofread/ add new nucleotides continuously
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13
Q

which strand uses DNA polymerase

A

leading strand

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14
Q

how is the leading strand synthesized

A
  • continously
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15
Q

which strand has its direction as 3’-5’

A

lagging strand

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16
Q

what makes the lagging strand unique

A
  • synthesized in short fragments
  • not recognized by DNA polymerase
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17
Q

which enzyme in a lagging strand prepares RNA primer so the DNA polymerase can recognize it

A

RNA polymerase

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18
Q

how does DNA polymerase add nucleotides in the lagging strand

A

discontinuously in short fragments called okazaki fragments

19
Q

function of DNA ligase

A

fills in the Okazaki fragments

20
Q

info in dna is copied as RNA nucleotides sequences to produce mRNA is known as

A

transcription

21
Q

what is copied in transcription

A
  • segment of DNA known as a gene
22
Q

what is a promotor

A

site where RNA polymerase binds to DNA marking the beginning on transcription

23
Q

which DNA strand is used in transcription

A

5’-3’

24
Q

in which type of microbes does transcription occur in the nuclues

A

eurkaroytes

25
Q

where does transcription occurs in prokaryotes

A

cytoplasm

26
Q

what allows RNA to exit the nuclues

A
  • single strand
  • RNA splicing
27
Q

what happens in the process of RNA splicing

A

introns are cut away and exons join

28
Q

function of exons

A

expressed region of DNA found on RNA

29
Q

what is the unexpressed region of DNA on RNA

A

introns

30
Q

which enzymes helps with RNA splicing

A

ribozyme

31
Q

does RNA splicing occur in prokaryotes

A

no

32
Q

function of translation

A

making proteins from mRNA

33
Q

condon is

A

3 nucleotides that code for 1 a.a

34
Q

AUG is

A

universla start codon

35
Q

universal stop aka nonsense condons are

A

UAA, UAG, UGA

36
Q

function of tRNA

A

carry a,a on acceptor arm

37
Q

which part of the tRNA carries an anticodon

A

long arm

38
Q

what is the site of translation

A

rRNA / ribosomes

39
Q

which direction does translation occur

A

5’-3’

40
Q

how does protein synthesis occur

A

2 subunits of ribosomes joining

41
Q

when can translation begin before transcription is done and why

A

in prokaryotes cuz transcription occurs in plasma

42
Q

what determines how a nucleotide sequence is converted to a.a

A

genetic code

43
Q

how many codons code for 20 a.a

A

64

44
Q

what determines the type of aminio acid that will be produced

A

codons on mRNA