Chemical Tests (Glycosides)

Question 1

Test for glycoside
observation: Reddish brown color
conclusion: 2-deoxysugars

Answer 1

Keller-Killiani Test

Question 2

Test for glycoside
conclusion: anthraquinone

Answer 2

Borntrager’s test

Question 3

Test for glycoside
observation: Honeycomb froth that persists for 10 mins
conclusion: Saponin

Answer 3

Froth test

Question 4

Test for glycoside
conclusion: saponin

Answer 4

Capillary test

Question 5

Test for glycoside
observation:
-α hemolysis – partial hemolysis
-β hemolysis – complete hemolysis
-γ hemolysis – no hemolysis
conclusion: Saponin

Answer 5

BAM (Blood Agar Media) Testor Hemolysis
Test

Question 6

Test for glycoside
observation: Brick Red / Red Coloration
conclusion: Cyanophore

Answer 6

Guinards Test

Question 7

Test for glycoside
observation: Rose pink to red
conclusion: Aloe emodin, barbaloin

Answer 7

Modified Borntrager’s test (anthraquinone)

Question 8

Test for glycoside
observation: Blue or violet color
conclusion: Cardiac glycosides

Answer 8

Antimony Trichloride Test

Question 9

Test for glycoside
observation: Green or bluish green color
conclusion: Cardiac glycosides

Answer 9

Liebermann Burchard test

Question 10

Test for glycoside
observation: Pale yellow ppt
conclusion: Anthraquinone
glycosides

Answer 10

Borax test or Schontetens Reaction

Question 11

Test for glycoside
observation: Yellow precipitate
conclusion: Flavonoid glycosides

Answer 11

Lead acetate solution

Question 12

Test for glycoside
observation: Red color on paper
conclusion: Cyanophore glycosides

Answer 12

Picrate paper