Chemical Pathology 15 - Enzymes and cardiac markers Flashcards

1
Q

What is the Km?

A

Michaelis Menten constant

= concentration of substrate at which the reaction velocity is 50% of maximum

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2
Q

What tumour marker can be used for germ cell, ovarian and testicular malignancies?

A

ALP

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3
Q

When is ALP elevated physiologically?

A

In pregnancy and childhood

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4
Q

Recall 4 pathologies that can cause a raised ALP

A
  1. Bile duct pathology (eg cholestatic liver disease)
  2. Bone pathology (fracture/ metabolic bone disease)
  3. Germ cell tumours
  4. Intestinal pathology (but not routinely used for investigation of bowel disorders)
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5
Q

Which LFT enzyme is most specific to liver pathology?

A

ALT

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6
Q

How can hepatic and biliary disease be best distinguished on the LFTs?

A

ALT:ALP ratio

If ALT much higher than ALP = hepatic

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7
Q

How many isoenzymes of LDH exist?

A

5

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8
Q

Recall 3 things that a raised LDH may indicate

A
  1. Haemolytic anaemia
  2. Tumour marker (lymphoma, germ cell testicular Ca)
  3. Myositis (rheum)
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9
Q

Which enzyme is markedly raised in acute pancreatitis?

A

Serum amylase

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10
Q

What is the most likely cause of a raised CK-MB1?

A

Rhabdomyolysis (history of long lie/ big fall?)

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11
Q

Where is CK-MB1 vs CK-MB2 produced?

A

CK-MB1 - skeletal muscle

CK-MB2 - cardiac muscle

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12
Q

In which population is CK physiologically slightly raised?

A

Afrocaribbean

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13
Q

Which prescription drug may be the cause of a raised CK-MB1?

A

Statins (as they can cause myopathy)

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14
Q

When does troponin I peak following chest pain?

A

2-4 hours

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15
Q

Within what time period does troponin I return to normal following a cardiac event?

A

5-10 days

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16
Q

What change in troponin levels is indicative of cardiac myocyte injury?

A

A 50% increase or decrease between 2 measurements