CHEMICAL EXAMINATION OF URINE Flashcards
memorization
Urine strip parameter(s) with 30 secs reading time
- Glucose
- Bilirubin
Urine strip parameter(s) with 40 secs reading time
- Ketones
Urine strip parameter(s) with 45 secs reading time
- Specific gravity
Urine strip parameter(s) with 60 secs reading time
- Protein
- pH
- Blood
- Urobilinogen
- Nitrite
Urine strip parameter(s) with 120 secs reading time
- Leukocyte esterase
Principle of Glucose urine strip
Double sequential enzyme reaction
Principle of Bilirubin urine strip
Diazo reaction
Principle of Ketones urine strip
Sodium nitroprusside reaction
Principle of Specific Gravity Urine Strip
pKa change of a polyelectrolytes
Principle of Protein urine strip
Protein error of indicators
Principle of pH urine strip
Double indicator system
Principle of Blood urine strip
Pseudoperoxidase activity of hemoglobin
Principle of urobilinogen urine strip
Ehrlich reaction
Principle of Nitrite urine strip
Greiss reaction
Principle of Leukocytes urine strip
Leukocyte esterase
Reagent Strip technique (true or false)
Dip the reagent strip briefly (no longer than 1 second) into a centrifuged urine specimen at RT.
false - dip the reagent strip into a will-mixed UNCENTRIFUGED urine specimen
Reagent Strip technique (true or false)
Remove excess urine by touching the edge of the strip to the container as the strip is withdrawn
True
Reagent Strip technique (true or false)
Blot the underside of the strip on a disposable absorbent pad
False - Blot the EDGE OF THE STRIP on a disposable absorbent pad
Reagent Strip technique (true or false)
Hold the strip vertically, and compare the color reaction of the strip pads to the manufacturer’s color chart in good lighting
False - Hold the strip HORIZONTALLY
true or false
Store without desiccant in an opaque, tightly closed container
False - Store WITH DESICCANT in an opaque, tightly closed container
true or false
Store below 30C (room temperature); do not freeze
True
Principle of automated reagent strip readers
Reflectance photometry
A measure of the amount of dissolved substances in a solution; Density of solution compared with density of similar volume of distilled water at a similar temperature
Specific Gravity
Reference ranges of specific gravity:
Random urine: __________
1st morning: __________
24-hour urine: _________
Random urine: 1.003 to 1.035
1st morning: >1.020
24-hour urine: 1.016 - 1.022
if S.G. is <1.003:
Not a urine n(except in D.I)
if S.G. is >1.040
Radiographic dye present (if refractometer is used)
S.G of:
Isosthenuria:
Hyposthenuria:
Hypersthenuria:
Isosthenuria: = 1.010
Hyposthenuria: < 1.010
Hypersthenuria: > 1.010
Calibration temperature of Urinometry (urinometer)
20C
______ for every 3C that the specimen temperature is below the calibration temp.
- 0.001 for every 3C that the specimen temp. is below the calibration temp.
______ for every 3C that the specimen temperature is above the calibration temp.
+ 0.001 for every 3C that the specimen temperature is above the calibration temp.
Correction for glucose and protein:
1 g/dL Glucose = __________
1g/dL Protein = _________
1 g/dL Glucose = -0.004
1g/dL Protein = -0.003
Urine volume required for urinometer:
10 - 15 mL
Principle or device for specific gravity measurement that doesn’t need temperature correction; requires correction for glucose and protein.
Refractometry (Refractometer, Rf/ TS (total solids) meter)
Calibration for refractometer:
Distilled/deionized H2O =
S.G. 1.000
Calibration for refractometer:
3% NaCl:
1.015 +/- 0.001
Calibration for refractometer:
5% NaC:
1.022 +/- 0.001
Calibration for refractometer:
7% NaCl:
1.035 +/- 0.001
Calibration for refractometer:
9% Sucrose
1.034 +/- 0.001
Sample problem: (URINOMETRY)
Urine S.G. reading by urinometer is 1.025
Urine temperature is 26C
Urinometer calibration temp. is 20C
what is the corrected SG?
1.027
Sample problem: (REFRACTOMETRY)
Urine S.G. reading by refractometer is 1.025 with 2 g/dL glucose and 2 g/dL protein
Temperature: 37C
what is the corrected SG?
1.011
Reagent for the rgt. strip reaction for specific gravity
bromthymol blue (multistix & chemstrip)
Obsolete method; based on frequency of soundwave entering a solution. changes in proportion to the density of the solution.
Harmonic oscillation densitometry (H.O.D)
Example of H.O.D
Yellow IRIS (International Remote Imaging System)
Urine volume required for IRIS diagnostics
6 mL urine volume
how much urine volume from the 6 mL is required for the IRIS mass Gravity meter?
2 mL (of 6mL)
how much volume from the 6 mL is required for IRIS Slideless microscope?
4 mL (of 6 mL)
Acidity refers to the “_______” of a solution, whereas alkalinity refers to its “_______”
Acidity: sourness
Alkalinity: bitterness
Normal urine pH:
Random urine:
1st morning:
Random urine: 4.5 - 8.0
1st morning: 5.0 - 6.0
When urine pH is >9.0:
unpreserved urine
occurs after meals due to the withdrawal of H+ ions for the purpose of secretion of HCl.
Alkaline tide
Causes of acid urine:
- Diabetes Mellitus (high ketone bodies)
- Starvation (high ketone bodies)
- High protein diet
- Cranberry juice (quinic acid–.Hippuric acid)
Causes of alkaline urine:
- Renal tubular acidosis
- Vegetarian diet
- After meal (alkaline tide)
- Vomiting
Reagent for the rgt. strip reaction for pH
Methyl red
bromthymol blue
interferences of reagent strip reaction for pH
No known interference
Runover from adjacent pads, old specimens
The parameter that is the most indicative of renal disease; produces white foam in urine when shaken
Protein
Normal urinary protein:
<10 mg/dL or <100 mg/day (Strasinger)
<150 mg/day (Henry)
Proteins in normal urine consist of ____ albumin and ____ globulins
1/3 albumin
2/3 globulins
Most abundant protein in normal urine
Tamm-Horsfall protein (Uromodulin)
Caused by conditions that affect the plasma prior to its reaching the kidney
Pre-renal (before) or Overflow proteinuria