chemical examination Flashcards
what is the purpose of reagent strip tests
to measure medically significant substances in urine
list issues associated with strip testing
- improper technique = result errors
- not mixed urine sample
- strip left in urine too long/not blotted
- not timing the reagents correctly
- reading of color charts
urine requirments for strip tests
- room temp
- well mixed
- free of contamination
- not thick specimen
list procedure steps of reagent strip testing
- check labels and mix
- pour into kova tube
- dip stick into kiva tube
- remove strip and touch edge to tube while removing (removes excess liquid)
- blot edges on paper towel
- wait specified time
- compare color changes on strip to color chart
describe handling requirments of strip tests
- protested from moisture, heat, light and chemicals (deterioration)
- desiccant remains in container
- container resealed immedietly
when is QC rub with strip tests
- 2 controls
- once per shift
- synthetic or batched urine (not distilled water)
define alkaline tide
urine is more alkaline throughout the day
what is normal urine pH
6-7 (greater than 9 = do not test)
clinical significance of pH
- rule out or in metabolic/respiratory acidosis or alkalosis (urine matches disorder)
- renaltubular secretion or reabs issues
- crystal ID
- kidney stone formation
- UTI treatment
how is pH indicated on a strip test
- methyl red pH 4-6
- bromothymol blue pH 6-9
name th emost indicative result of renal disease
protein determination
- early renal disease
what protein does the strip test detect
albumin
name other proteins found in urine
- serum/tubular microglobulins
- Tamm-Horsfall protein (uromodulin): tubular protein
- prostatic/seminal/vaginal secretions
what causes the indicator to change colors in protein strip testing
protein acceptance of hydrogen in acid system (pH=3)
name the protein indicator dye
tetrabromphenol blue
