Chem Flashcards
If the pressure remains constant, then the volume must increase.
The ideal gas law states that PV = nRT. If n (the quantity of gas) is constant and T (temperature) increases, then PV (pressure × volume) will increase.
Which of the following compounds is a weak electrolyte?
BaCl
CH3OH
KOH
CH3COOH
CH3COOH –> Weak electrolyte
KOH and BaCl –> Strong electrolyte
CH3OH –> non-electrolyte
H3O+
Lewis acid
Write out Kw, pH, pOH, and pKw
Rank the colors of visible light in order from lowest frequency to highest.
By frequency Red< orange< yellow< green< blue< violet
Rank in order of wave length, from shortest to longest, the regions of the EM spectrum.
By wavelength, gamma rays <x-rays< ultraviolet< visible light< infrared< microwaves< radio waves.
What are the key characteristics about photons an electron energy levels?
- A product of the wavelength and frequency of a photon is equal to 3.0 x 10^8 m/s
- The difference in energy between adjacent energy levels decreases as the energy level increase
- Energy of a photon is directly proportional to its frequency
Visible light
ROYGBIV
400-700
Kcat
Absorption of ultraviolet light by organic molecules always results in what process?
Excitation of bound electrons
The half-life of a radioactive material is:
the time it takes for half of all the radioactive nuclei to decay into their daughter nuclei.
Which property of a substance is best used to estimate its relative vapor pressure?
Boiling point
What are the structural features possessed by storage lipids?
Three fatty acids ester-linked to a single glycerol
It is possible to design a reactor where the SCY conductor and the nitrogen/ammonia electrode operate at different temperatures. Which combination of temperatures is expected to give the best results?
Conductor temperature higher than electrode temperature
Coordinate covalent
Lewis acid–base interaction between a metal cation and an electron pair donor is known as a coordinate covalent bond
Least polar bonding
bonds between identical atoms are likely to be the least polar
Which of the following energy conversions best describes what takes place in a battery-powered resistive circuit when the current is flowing?
Chemical to electric to thermal
Protein secondary structure is characterized by the pattern of hydrogen bonds between:
backbone amide protons and carbonyl oxygens.
Ubiquitination
targets a protein for degradation by a proteasome.
Glycolysis
Electrophoresis
Purpose:
* separate things (DNA, RNA, proteins) by size
* separate proteins by isoelectric point
Southern Blot
detect specific sequences of DNA
Northern blotting
Northern blotting:
to detect specific sequences of RNA
-usually used to determine expression, since you are
analyzing the mRNAs in a cell
-still use a DNA probe
Western Blot
to detect the presence of specific proteins in a sample
-sample can be isolated from specific cell types, or
blood, etc.
-can also determine size of protein
How to use:
1. Use a primary antibody, specific for your protein of interest,
to “find” the protein on the filter
- Use a secondary antibody, specific to the primary antibody,
that has been tagged with an enzyme that will fluoresce
when it encounters its substrate - Expose the filter to the substrate to trigger fluorescence to
visualize the protein
Eastern Blot
to detect posttranslational modifications on proteins
ELISA
to detect the presence of specific proteins in a sample
-detect antigen (e.g., bacterial surface proteins, viral
proteins, etc.) or antibodies
-similar to Western blot, but better info on amount of
protein in sample