Chapter Six Flashcards
What are the 3 main properties of a microscope that influence the image?
magnification, resultion, contrast
What is magnification?
ratio of image size actual size; multiplication factor of size
What is resolution?
minimum distance two points can be separated and still be distinguished as separate points; image clarity
What is contrast?
difference in brightness between light and dark areas of an image
What are the 3 main types of microscopes?
compound light microscope, disseecting/stereo microscope, electron microscope
Which microscope inverts an image?
compound light microscope
How many SETS of lenses does a compound light microscope use to magnify an image?
2
Which microscope requires a specimen to be stained before use? Why?
compound light microscope; since cells are usually transparent
What is the magnification of a compound light microscope?
400x (1000x with oil immersion)
What is the best resolution of a compound light microscope?
0.2 micrometers
What view (of a sample) does a compound light microscope provide?
thin 2D view of sample
What is the magnification of a dissecting/stereo microscope?
20x-80x
What view (of a sample) does a dissecting/stereo microscope provide?
3D view of sample
What are the two types of electron microscopes (EM)?
scanning EM, transmission EM
In a scanning EM, electrons move ____ ___ _____ across a specimen
back and forth
Scanning EM renders details by __________
reflection
In a transmission EM, electron beams are….
transmitted through a specimen
Transmission EM provides….
details of internal structures
Electron microscopes have a ______ magnification and resolution
higher
What are the three domains of living organisms?
archaea, bacteria, eukarya
What are archaea?
single celled organisms without a nucleus, extremophiles, prokaryotes
formerly grouped with bacteria
What are bacteria?
all other prokaryotes aside from archaea
What are the kingdoms of eukarya?
animalia, plantae, fungi (FORMERLY protists)
What are cells?
basic unit of structure and function in a living organism
Prokaryotes are generally _______ than eukaryotes. Why?
smaller; prokaryotes are single-celled
Prokaryotes or Eukaryotes: Which has a nucleus?
Eukaryotes
Prokaryotes or Eukaryotes: Which has organelles?
Eukaryotes
What are the 4 common components of all cells?
plasma membrane, cytoplasm, chromosomes, ribosomes
What is the plasma membrane?
outer covering separating the
cells interior from its surroundings
What is the cytoplasm?
area between cell membrane, nucleus, and all components within this area
largely filled with cytosol: jellylike