chapter 9 principles of serology testing Flashcards
dilutions, serial dilutions, pipettes, titers
Study of fluid components in the blood, especially antibodies
Serology
Liquid portion of blood minus coagulation factors
Serum
the sample most used in Serology
Serum
contains all the clotting
factors and fibrinogen
plasma
Fresh serum that has not been heated
best testing method
What’s the storage for serology tests?
Between 2°C and 8°C for up to 72 hours, if delay in test freeze sample 20 c or below
what can cause false positives?
hemolysis
used to measure specimen for testing or liquid for making reagents and dilutions
pipettes
volumetric pipettes
single volume, precise delivery
graduated pipettes
can deliver varied volumes
micropipettes
smaller volume, more accurate, uL
marked and calibrated to deliver one volume
volumetric pipette
which pipette is ‘TD’ to deliver?
volumetric pipettes
which pipette is ‘TC’ to contain?
graduated pipettes
has marks along its length and can deliver varied volumes
graduated pipettes
measuring pipette that has marks along its lengh down to the tip, last drop must be “blown out”
serological pipettes
a serial dilution of a pt serum adding ag/ab to observe for reactivity
antibody titer
acute sample
pt having known symptoms
convalescent sample
pt is recovering
what is a dx ab titer
x4 rise in ab titer from previous measurement
