ch. 11 immunoassays

Question 1

the ability to detect very small
amounts of a substance

Answer 1

Analytical Sensitivity

Question 2

the ability to detect a
substance without interference from cross-reactivity
substance

Answer 2

Analytical Specificity

Question 3

substance being measured; can be an
antigen or antibody

Answer 3

Analyte

Question 4

why use labeled immunoassays

Answer 4

to detect small or low concentrations of analyte

Question 5

assays in which the patient
analyte and labeled reagent
compete for a limited
number of binding sites on
reagent antibody.

Answer 5

Competitive Immunoassays

Question 6

Patient antigen is captured by antibody bound to a solid phase. After washing to remove unbound antigen, a second antibody with a label is added to the reaction.The amount of label is directly
proportional to the amount of antigen in the patient sample.

Answer 6

Noncompetitive Immunoassays, also known as Capture, Sandwich,
or immunometric assays

Question 7

Excess patient antigen
causes falsely decreased
detection (post zone)

Answer 7

high dose effect

Question 8

patient ab produced in vivo that resembles reagent ab

Answer 8

autoantibodies

Question 9

antibodies that react with animal protein

Answer 9

heterophile antibody

Question 10

detection of a
substance other than the analyte of interest, typically observed as a false positive

Answer 10

cross reactivity

Question 11

Membrane-based, single-use assays based on immunochromatography, fast turnaround time;
ideal for point-of-care testing

Answer 11

rapid immunochromatographic