Chapter 9: Gram-negative rods: Enterics Flashcards
What does EMB agar stand for?
Eosine Methylene Blue
What is the Gram stain and morphology of the enterics?
Gram-negative rods
What type of bacteria grow on EMB agar?
Gram-negatives
EMB agar inhibits the growth of which type of bacteria?
Gram-positives
True or False: Gram-positives can grow on EMB agar.
False
True or False: EMB agar inhibits the growth of Gram-positive bacteria.
True
True or False: Gram-negatives can grow on EMB agar.
True
True or False: EMB agar inhibits the growth of Gram-negative bacteria.
False
EMB agar is used to detect what type of Gram-negative bacteria?
lactose-fermenters
What color do lactose fermenters have when grown on EMB agar?
become deep purple to black
Lactose fermenting Gram-negative bacteria appear purple to black when grown on what growth media?
EMB agar
What type of bacteria appear purple to black when grown on EMB agar?
Gram-negatives: lactose fermenters
What bacteria appear purple on EMB agar with a metallic green sheen?
Escherichia coli
Escherichia coli colonies appear purple with a metallic green sheen when grown in what media?
EMB agar
What 2 main types of media inhibit the growth of Gram-positive bacteria (so only Gram-negative bacteria grow on it), and have indicators that change color in the presence of lactose fermentation?
1) EMB agar
2) MacConkey agar
What type of bacteria grow on MacConkey agar?
Gram-negatives
MacConkey agar inhibits the growth of what type of bacteria?
Gram-positives
True or False: Gram-positives can grow on MacConkey agar.
False
True or False: Gram-negatives can grow on MacConkey agar.
True
True or False: MacConkey agar inhibits the growth of Gram-negatives.
False
True or False: MacConkey agar inhibits the growth of Gram-positive bacteria.
True
What color do lactose fermenters have when grown on MacConkey agar?
Pink/purple
Lactose-fermenting Gram-negative bacteria appear pink/purple when grown on what growth media?
MacConkey agar
What components of MacConkey agar inhibit the growth of Gram-positive bacteria? (2)
1) bile salts
2) crystal violet