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MTAP 1 > Chapter 8 - Mahon > Flashcards

Chapter 8 - Mahon Flashcards

1
Q

Key ingredients of Anaerobic blood agar

A

intact sheep RBCs
vitamin K
yeast extract

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2
Q

Use of anaerobic blood agar

A

isolation and subculturing obligate anaerobes

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3
Q

key ingredients of CHOC agar

A

hemolyzed sheep RBC
hemoglobin
NAD

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4
Q

CHOC agar use

A

primary plating and subculture fastidious bacteria (Haemophilus, Neisseria)

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5
Q

key ingredients of Hektoen enteric agar

A

carbohydrates (lactose, salicin, sucrose)
bile salts (inhibits gram + and nonpathogenic enteric bacteria)

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6
Q

selective and differential medium for primary plating of stool spx to recover intestinal pathogens (Salmonella)

A

Hektoen enteric agar

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7
Q

MAC agar key ingredients

A

lactose
low conc. bile salts (inhibits gram + but permits growth of gram -)

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8
Q

selective and differential medium for isolation of gram - bacteria; used for primary plating and subculturing

A

MAC

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9
Q

modified thayer martin ingredients

A

choc agar base
antimicrobial agents (vancomycin, colistin, trimethoprim, nystatin)

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10
Q

selective primary plating medium for the recovery of Neisseria gonorrheae and N. meningitidis

A

MTM

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11
Q

SDA ingredients

A

dextrose (glu)
antimicrobials to inhibit bacteria

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12
Q

SBA ingredients

A

intact RBCs

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13
Q

primary plating and subculturing of most bacterial isolates

A

SBA

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14
Q

T/F

Organisms that grow on CHOC also grow on SBA. But not all organisms that grow on CHOC will grow on SBA.

A

T

CHOC allows growth of fastidious organisms (highly fastidious - Haemophilus, Neisseria)

These organisms will not grown on SBA

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15
Q

growth on SBA and CHOC but not on MAC

A

indicative of:

gram + or fastidious gram - bacillus or coccus

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16
Q

best used to characterize gram - rods because lactose fermenters can be differentiated from lactose nonfermenters

A

MAC

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17
Q

colonies of lactose fermenters in MAC

A

pink, dark pink, or red colonies
(as pH decreases)

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18
Q

colonies of nonlactose fermenters in MAC

A

colorless

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19
Q

differentiation of lactose to nonlactose fermenters screens for

A

enteric pathogens from stool culture (most are NON LACTOSE FERMENTERS)

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20
Q

partial lysing of RBCs in SBA around and under the colony that results in GREEN discoloration

A

a-hemolysis

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21
Q

ex of organisms that produce a-hemolysis

A

s. pneumoniae
viridans strep

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22
Q

complete clearing of RBCs in SBA

A

b-hemolysis

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23
Q

produces a wide, deep, clear zone of b-hemolysis

A

group A b-hemolytic strep
(S. pyogenes)

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24
Q

produces a narrow, diffuse zone of b-hemolysis close to the colony

A

group B b-hemolytic strep (S. agalactiae)
L. monocytogenes (gram + rod)

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25
Q

a visual comparison between genera and species

A

size of colonies

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26
Q

description of colony size

A

large
medium
small
pinpoint

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27
Q

description of colony margin

A

smooth
rough or rhizoid
filamentous
irregular

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28
Q

with filamentous appearance, described as Medusa heads

A

Bacillus anthracis colonies

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29
Q

ex of genera that may swarm in blood and CHOC agar

A

Proteus (esp. mirabilis)

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30
Q

hazy blanket of growth on the surface that extends well beyond the streak lines

A

swarming

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31
Q

produce colonies that have a dry appearance and umbonate center growing on SBA

A

diphtheroids

32
Q

produce colonies that are creamy, white with dull surface, with feet/pedicles

A

yeasts

33
Q

produce moist, creamy white to yellowish colonies

A

Staphylococci

34
Q

description of colony elevation

A

raised
convex
flat
umbilicate (depressed center/concave)
umbonate (raised/bulging center)

35
Q

bacteria typically produces umbilicate colonies

A

S. pneumoniae

36
Q

bacteria typically produces convex colonies

A

S. aureus

37
Q

produces flat colonies

A

b-hemolytic strep

38
Q

description of colony density

A

transparent
translucent
opaque

39
Q

have semi opaque colonies with the organism concentrated at the center of the colony

bull’s eye colony

A

group B (S. agalactiae)

40
Q

shiny, similar to half-pearl colony in blood containing media

A

B. pertussis

41
Q

term used to describe a particular GENUS in general

A

color

42
Q

with white colonies

A

coagulase-neg staph

43
Q

with yellow or off-white colonies

A

micrococcus
neisseria (non pathogenic)

44
Q

with gray colonies on SBA

A

gram negative rods

45
Q

detemined by touching the colony with sterile loop

A

consistency

46
Q

description of colony consistency

A

brittle (splinters)
creamy (butyrous)
dry
waxy
sticky

47
Q

with creamy colonies

A

S. aureus

48
Q

with sticky colonies

A

certain Neisseria

49
Q

with brittle, crumbly, and wrinkled colonies

A

Nocardia

50
Q

with dry colonies (except for mucoid types) wherein when pushed by a loop, whole colony remains intact

A

beta hemolytic strep

51
Q

inherent characteristics of a specific organism confined generally to the colony

A

pigment

52
Q

how is pigment production enhanced

A

grow bacteria at ROOM TEMP

53
Q

with green, sometimes metallic sheen pigment

A

P. aeruginosa

54
Q

with brick red pigment, esp. at RT

A

Serratia rubidaea

55
Q

with blue pigment

A

Kluyvera

56
Q

with purple pigment

A

C. violaceum

57
Q

with brown to black (anaerobic) pigment

A

P. melaninogenica

58
Q

with old sock odor (evident when grown on mannitol salt agar)

A

S. aureus

59
Q

with fruity or grapelike odor

A

P. aeruginosa

60
Q

with putrid odor

A

P. mirabilis

61
Q

with musty basement, mousy or mouse nest smell

A

Haemophilus spp.

62
Q

with freshly plowed field odor

A

Nocardia spp.

63
Q

forms a large, rough, greenish, hemolytic colonies on SBA

A

B. cereus

64
Q

forms a small fuzzy edged colony with an umbonate center on SBA or choc

A

Eikenella corrodens

65
Q

translucent, may resemble water droplet
umbilicate, or flat w/ “penny” edge
entire margin, wide and strong zone of alpha hemolysis

A

S. pneumoniae

66
Q

translucent, grayer, rougher margin, umbonate center

A

a-hemolytic viridans strep

67
Q

pinpoint brittle translucent
gray that may turn brownish on continued incubation

large deep zone of b-hemolysis

A

S. pyogenes

68
Q

medium sized colony compared to S. pyogenes
creamy, gray, small, diffuse zone of b-hemolysis
often need to remove colony with a loop to see b-hemolysis

bull’s eye colony

A

S. agalactiae

69
Q

large, flat, convex, or umbonate center after 24hr

shiny, moist, creamy, white to offwhite

A

Staphylococcus

70
Q

smaller than staphylococci
convex, grows upward more than outward
creamy, white, dull surface
tiny projections at the base of colony after 24hr

A

Candida albicans (yeast)

71
Q

exhibits vine or streamer (more prevalent toward the tube bottom) in liquid media

A

streptococci

72
Q

exhibits puffed balls in liquid media

A

streptococcal spp

73
Q

produce “scum” at the surface of thioglycollate

A

yeast

74
Q

produce turbidity when growing in thioglycollate. gas bubbles at the surface and middle

A

enterics

75
Q

produce “scum” at the sides of thioglycollate with diffusable green pigment and metallic sheen at the surface

A

pseudomonas spp

MTAP 1 (13 decks)

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