Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

MTAP 1 > AUBF (reagent strip) > Flashcards

AUBF (reagent strip) Flashcards

1
Q

reading time: BILIRUBIN

A

multistix: 30s
chemstrip: 60s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Principle: BILIRUBIN

A

Diazo reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Multistix sensitivity: BILIRUBIN

A

0.4-0.8 mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Chemstrip sensitivity: BILIRUBIN

A

0.5 mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Multistix reagent: BILIRUBIN

A

2,4 - Dichloroaniline diazoniun salt

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Chemstrip reagent: BILIRUBIN

A

2,6 - Dichlorobenzene diazoniun salt

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

BILIRUBIN RXN

A

bilirubin glucuronide + diazonium salt
—(acid)—> azodye

dichloroaniline end-color: tan
dichlorobenzene end-color: pink-violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

type of bilirubin that appears in urine

A

conjugated

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Associated with BILIRUBINURIA

A

hepatitis
cirrhosis
gallstones
carcinoma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

correlation to BILIRUBIN

A

urobilinogen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

false (+) sources of BILIRUBIN

A

• pigmented spx
• Phenazopyridine (gives yellow-orange color)
• Lodine metabolites
• Indican (intestinal disorder)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

False (–) BILIRUBIN

A

• Light exposure (photo oxidized to biliverdin that does not react with Diazo)
• Ascorbic acid (>25 mg/dL)
• Inc. urine concentration
• Old spx
• Bilirubin hydrolysis to diglucoronide —> free bilirubin
• Nitrite (attaches to diazonium salt)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Confirmatory test for Bilirubin

A

Ictotest tablets
Result (+): blue —> purple color

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

screening test for DM

A

glucose chem strip

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

elevated glucose level

A

hyperglycemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

glucose renal threshold

A

160-180 mg/dL
(blood level at which tubular reabsorption stops)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

fluctuating blood glucose level on a NONFASTING normal person after a meal

A

glycosuria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

fasting spx required

A

2nd morning specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

hyperglycemia during pregnancy

A

gestational diabetes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

glycosuria in absence of hyperglycemia during malfunction in reabsorption of glucose by renal tubules

A

renal glycosuria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

GLUCOSE reading time

A

30s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

GLUCOSE principle

A

Double sequential enzymatic rxn
(glucose oxidase, peroxidase)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Glucose multistix sensitivity

A

75-125 mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Glucose Chemstrip sensitivity

A

40 mg/dL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

reagents for GLU multistix
end color

A

glu oxidase, peroxidase
POTASSIUM IODIDE (green to brown)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

reagents for GLU chemstrip
end color

A

glu oxidase, peroxidase
TETRAMETHYLBENZIDINE (yellow to green)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

other examples of chromogens for glu rxn

A

clinistix: O-toluidine (pink—> purple)
aminopropyl-carbazol (yellow —> orange-brown)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

correlation to GLUCOSE

A

ketone
protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

false (+) GLUCOSE

A

• oxidizing agents (contaminated cont with peroxide)
• detergents

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

false (–) GLUCOSE

A

• Increased Ascorbic acid, SG, Ketones
• Decreased temp.
• Improperly preserved spx standing at RT

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

remedy for increased ascorbic acid causing false neg GLUCOSE

A

iodate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

represents 3 intermediate products of fat metabolism

A

ketones

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

Ketones reading time

A

40s

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

Ketones principle

A

Sodium nitroprusside (nitroferricyanide) rxn

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

Ketones multistix sensitivity

A

5-10 mg/dL (acetoacetic acid)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

Ketones chemstrip sensitivity

A

9 mg/dL (acetoacetic acid)

70 mg/dL (acetone)

37
Q

Ketones multistix reagent

A

Sodium nitroprusside (detects acetoacetic acid)

38
Q

Ketones chemstrip reagent

A

Sodium nitroprusside + GLYCINE (detects acetoacetic acid + acetone)

39
Q

early indicator of insufficient dosage in type 1 diabetes

40
Q

correlated to ketones

41
Q

false (+) KETONES

A

• Levodopa
• Sulfhydril medications (Mercaptoethane sulfonate sodium [MESNA] and Captopril)
• Phthalein dyes
• Pigmented spx
• Improperly timed reading

42
Q

False (–) KETONES

A

Improperly preserved spx causing volatilization of acetone and acetoacetic acid

43
Q

SG reading time

44
Q

SG principle

A

pKa (dissociation constant) changes of polyelectrolyte in ALKALINE medium

45
Q

SG sensitivity

A

1.000-1.030

46
Q

color if SG is 1.000

A

blue (alk)

47
Q

color if SG is 1.030

A

yellow (acid)

48
Q

intermediate color if SG is betw. 1.000-1.030

A

shades of GREEN

49
Q

SG Multistix reagent

A

Poly (methylvinylether/maleic anhydride)
Indicator: bromthymol blue

50
Q

SG Chemstrip reagent

A

Ethyleneglycol diaminoethylether tetraacetic acid
Indicator: bromthymol blue

51
Q

T/F
Spx with pH of 6.5 or higher have decreased readings caused by interference with bromthymol blue

52
Q

False (+) SG

A

increased PROTEIN conc.

53
Q

False (–) SG

A

highly alkaline urine (equal or > pH 6.5)

54
Q

what is recommended if the pH of urine is equal or > pH 6.5 that may cause decreased SG readings?

A

add 0.005 to SG

55
Q

reagent for pH

A

Methyl red
Bromthymol blue

56
Q

pH MULTISTIX sensitivity

A

5.0 to 8.5 in 0.5 increments

57
Q

pH CHEMSTRIP sensitivity

A

5.0 to 9.0 in 1.0 increments

58
Q

Sources of error/ interference for pH

A

No known interfering substances

Run-over from adjacent pads (close to
protein pad - highly acidic)

Old specimens

59
Q

pH is correlated with these tests

A

Nitrite
Leukocytes
Microscopic

60
Q

MULTISTIX reagent for protein

A

Tetrabromophenol blue

61
Q

CHEMSTRIP reagent for protein

A

3’,3’’,5’,5’’-tetrachlorophenol
3,4,5,6-tetrabromosulfophthalein

62
Q

Protein MULTISTIX sensitivity

A

15 to 30 mg/dL albumin

63
Q

Protein CHEMSTRIP sensitivity

A

6 mg/dL albumin

64
Q

False (+) protein

A

Highly buffered interference alkaline urine

Pigmented specimens, phenazopyridine

Quaternary ammonium compounds (detergents)

Antiseptics, chlorhexidine

Loss of buffer from prolonged exposure of the strip to the specimen reagent

High specific gravity

65
Q

False (-) protein

A

Proteins other than albumin
Microalbuminuria

66
Q

Tests correlated with protein

A

Nitrite
Leukocytes
Microscopic

67
Q

MULTISTIX reagent for glucose

A

Glucose oxidase
Peroxidase
Potassium iodide

68
Q

CHEMSTRIP reagent for glucose

A

Glucose oxidase
Peroxidase
Tetramethylbenzidine

69
Q

MULTISTIX reagent for blood

A

Diisopropylbenzene dihydroperoxide

3,3’,5,5’- tetramethylbenzidine

70
Q

CHEMSTRIP reagent for blood

A

Dimethyldihydroperoxy-hexane

Tetramethylbenzidine

71
Q

Parameters affected by ascorbic acid causing FALSE NEG

A

Blood
Bilirubin
Leukocytes
Nitrite
Glucose

72
Q

Tests correlated with Blood

A

Protein
Microscopic

73
Q

MULTISTIX reagent for urobilinogen

A

p-dimethylaminobenzaldehyde

74
Q

CHEMSTRIP reagent for urobilinogen

A

4-methoxybenzene-diazonium- tetrafluoroborate

75
Q

Urobilinogen MULTISTIX sensitivity

A

0.2 mg/dL urobilinogen

76
Q

Urobilinogen CHEMSTRIP sensitivity

A

0.4 mg/dL urobilinogen

77
Q

MULTISTIX reagent for nitrite

A

p-arsanilic acid
Tetrahydrobenzo(h)-quinolin-3-ol

78
Q

CHEMSTRIP reagent for nitrite

A

Sulfanilamide, hydroxytetrahydro benzoquinoline

79
Q

MULTISTIX reagent for LE

A

Derivatized pyrrole amino acid ester
Diazonium salt

80
Q

CHEMSTRIP reagent for LE

A

Indoxylcarbonic acid ester
Diazonium salt

81
Q

reagent for Ascorbic acid

A

2,6-dichlorophenolindopheno

82
Q

pH principle

A

double indicator system of methyl red and bromothymol blue

83
Q

protein principle

A

protein (sorensen’s) error of indicator

84
Q

blood principle

A

pseudoperoxidase activity of Hgb

85
Q

urobilinogen principle

A

ehrlich’s rxn

86
Q

nitrite principle

A

greiss rxn

87
Q

LE principle

88
Q

ascorbic acid principle

A

reduces a dye in the pad, causing color change BLUE —> ORANGE

89
Q

blood stip results

speckled blue: ?
uniform blue: ?

A

speckled blue: intact RBC (hematuria) - cloudy red urine

uniform blue: hgb and myoglobin (hemoglobinuria) - clear red urine

MTAP 1 (13 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions