Chapter 6 - Culture Media

Question 1

________ aerobic growth and growth occurs at a high concentration of oxygen, located at the top of the test tube.

Answer 1

Obligate aerobes

Question 2

________ anaerobic growth and growth require absense of O2, located at the bottom of the test tube.

Answer 2

Obligate anaerobes

Question 3

_______ aerobic and anaerobic growth but prefers the presence of oxygen. Can grow throughout the test tube but greater growth is located at the top of the test tube.

Answer 3

Facultative anaerobes

Question 4

________ aerobic growth but requires a low concentration of oxygen. This is typically found around the center of the test tube.

Answer 4

Microaerophiles

Question 5

______ is the introduction of microbes into a medium.

Answer 5

Inoculum

Question 6

microbes growing in or on a culture medium is a ______.

Answer 6

Culture

Question 7

______ contains only one species seen on a solid agar plate.

Answer 7

Pure culture

Question 8

_______ is a population of cells from a single cell or from a group of attached cells.

Answer 8

Colony

Question 9

The exact composition of a __________ is known.

Answer 9

Chemically Defined Media

Question 10

The chemical composition of _______ varies from batch to batch and the exact composition is unknown.

Answer 10

Complex Media

Question 11

All available O2 is removed from ______, meaning that it is solely for anaerobic bacteria growth.

Answer 11

Reducing Media

Question 12

_________ contains chemicals that combine with O2 to deplete it. An example of ________ is an anaerobic jar.

Answer 12

Reducing Media

Question 12

Blood agar allows for differentiation between bacteria within the same group. This is an example of ________. Some media can have both selective and differential characteristics, such as EMB agar

Answer 12

Differential Media

Question 13

_______ is selective for gram-negative bacteria only and suppresses against gram-positive.

Answer 13

Selective Media

Question 13

________ suppresses unwanted microbes and promotes the growth of the desired microbe. An example of _______ is EMB (Eosin Methylene Blue) agar.

Answer 13

Selective Media

Question 14

_______ allows for the growth of many bacterial species. It can even grow fastidious bacteria, which are known as “picky” and require many growth factors. It usually exists as a liquid.

Answer 14

Enrichment Media

Question 15

Microbial growth is determined in the lab by observation of a ____________.

Answer 15

culture medium

Question 16

The ___________ is used to isolate pure bacterial cultures

Answer 16

streak plate method

Question 17

_____________ is the time required for a cell to divide (double). It usually ranges from minutes to hours

Answer 17

generation time

Question 18

__________ is the increase in the number of cells, not the cell size. This is done by a process called ___________.

Answer 18

bacterial division
binary fission

Question 19

Bacterial growth is represented by ________________

Answer 19

growth curves

Question 20

A device called a(n) __________ is used to measure cloudiness/turbitity

Answer 20

spectrophotometer