Chapter 5.1 : How to Interpret Tissue Sections Using a Microscope

Question 1

Microscopic sectioning changes a ______structure
into a _______

Answer 1

three-dimensional structure
into a two-dimensional image

Question 2

Preparation of histological specimens: Fixate, slice, and stain

– fixative prevents ____ , but in process
dissolves and removes the ___ from the tissue
– histological sections – tissue is sliced into thin sections
_____ cells thick
– stains – tissue is mounted on slides and artificially colored
with histological stain - increases contrast // different
stains may be used to bind to different cellular components
to increases contrast

Answer 2

decay ; fat

one or two

Question 3

longitudinal section

Answer 3

tissue cut along long
direction of organ

Question 4

cross section or transverse
section

Answer 4

tissue cut perpendicular to
length of organ

Question 5

oblique section

Answer 5

tissue cut at angle between
cross and longitudinal
section