Chapter 5 Flashcards
During what stage in a cell cycle does DNA replicate?
interphase
What are the names of the 4 bases that form the “sequence” of DNA?
Adenine, Cytosine, Guanine, Thymine
What would the complementary DNA sequence be for this DNA single strand: CATATACGCATATAC ?
GTATATGCGTATATG
What would happen if you used one restriction enzyme to cut the DNA found at the crime scene and different ones to cut the DNA of the suspects?
if you used one restriction enzyme at crime scene and a different one on the suspect, you would not find a matching sequence of DNA because the specific sequences you are looking for would be different.
What exactly did you need to see to determine if a suspect’s DNA matched the crime scene?
You needed a sample of DNA from the crime scene and a sample from the suspect and a gel electrophoresis apparatus. From there you ran the test to see of the DNA from the crime scene matched the suspects from the results of the gel electrophoresis.
Why do some DNA fragments migrate farther from their starting point than others in the same lane of the gel?
Smaller molecules move faster and larger molecules move slower, therefore smaller molecules are father away from their starting point and larger molecules are closer
- What do you think happens if you load too much DNA into some of the gel lanes (how does it affect your results, if at all)?
Too much of your DNA sample could cause it to smear or appear smaller than it truly is, tis would give you inaccurate or inconclusive results.
- What did you need to be able to see the bands of DNA?
Ultraviolet Light
- When a suspect’s DNA matches that of the crime scene, what does it mean?
Just because someone’s DNA was found at the scene does not prove guilt - only that they were at the scene. Other evidence may need to be provided to eliminate reasonable doubt.
