Chapter 4: Enzymes

Question 1

Outline the induced fit hypothesis.

Answer 1

1. Substrate binds to active site.
2. Active site changes shape.
3. Closer fit between active site and substrate.
4. More bonds between active site and substrate.
5. ESC forms.
6. Change in shape of active site weakens bonds in substrate.
7. Activation energy reduced.
8. Further shape change after products are released from active site.

Question 2

Outline the lock and key hypothesis.

Answer 2

1. Substrate complementary to active site.
2. Substrate binds to active site.
3. ESC forms.
4. Puts strain on bonds in substrate.
5. Enzyme-product complex forms.
6. Products/amino acids leave active site.