Chapter 21: Manipulating Genomes

Question 1

Outline role of PCR in sequencing a genome.

Answer 1

• Make copies of DNA.

- Range of different lengths.

Question 2

Outline role of electrophoresis in sequencing a genome.

Answer 2

• Put DNA pieces in size order.

- To read base sequence.

Question 3

Outline role of digestion of DNA by restriction enzymes in sequencing a genome.

Answer 3

• Cut genome into smaller/750 bp fragments.

- Cut vectors/BACs/plasmids for gene library.

Question 4

Suggest why genome has to be fragmented before sequencing.

Answer 4

• Genome too large.
• Accuracy better/fewer errors with small fragments.
• Divide job over time/different labs.