Chapter 28 Flashcards
The Italian physician who suggested that disease was caused by unseen organisms in 1546
Girolamo Fracatoro
The first to observe and accurately describe microbial life
Anton van Leeuwenhoek
French microbiologist who refuted the controversy over spontaneous generation
Louis Pasteur
German physician who studied anthrax and was able to determine the causative agent
Robert Koch
Koch’s postulates
- The microorganism must be present in ____ and absent in ____
- The causative agent must be ____
- The same disease must result when ___
- The same microorganisms must be ____
- must be present in every case of the disease and absent in healthy individuals
- Isolated and grown in a pure culture
- When the cultured microorganism is used to infect a healthy host
- Isolated again from the diseased host
The oldest, structurally simplest, and most abundant forms of life. The only organisms with prokaryotic cellular organization.
Archaea and bacteria
Unicellularity
Prokaryotes are fundamentally single celled
In their natural environments most bacteria appear to be capable of forming a complex community of different species called a ___
Biofilm
Cell size
Most prokaryotic cells are only 1 micrometer or less in diameter, they are very small
Nucleoid
Prokaryotes lack a membrane bound nucleus and instead usually have a singular circular chromosome
Flagella
Prokaryotic flagella are composed of a single fiber. Bacterial flagella are also more rigid and spin like a propellar
Metabolic diversity
Prokaryotes are the only chemolithoautotrophic organisms meaning they use inorganic molecules to make carbohydrates
Name the four key areas that Archaea and Bacteria differ
Plasma membranes
Cell wall
DNA replication
Gene expression
Archaen plasma membrane lipids are composed of _____ linked to ______ by ether linkages, not the ester linkages seen in bacteria and eukaryotes
glycerol linked to hydrocarbon chains by ether linkages
The cell wall of bacteria are constructed minimally of ___ which is formed from carbohydrate polymers linked together
Peptidoglycan
The cell walls of archaea lack peptidoglycan although some have ____ which is similar in structure and function
Pseudomurein
Archael initiation of DNA replication is similar to that of ___
eukaryotes
In terms of gene expression, archaea may have more than one ____. Some of the translation machinery is also more similar to that of eukaryotes
RNA polymerase
Molecular approaches to classification of Prokaryotes
- the analysis of ______ of key proteins
- The analysis of ____ by establishing a percent of G and C
- ______ the mixing of single-stranded DNA from two species and determining the amount of base pairing
- ___ and ____ sequencing, especially looking at ribosomal RNA
- Whole ____ sequencing
- the amino acid sequence of key proteins
- nucleic acid-base sequences by establishing the percent of G and C
- Nucleic acid hybridization
- gene and RNA
- genome
Developed the three-domain system of phylogeny
Carl Woese
The study of prokaryotic organisms was first made possible by
microscopes
Early microbiology grew from the study of
disease-causing agents
Bacteria and archaea are clearly different based on both ___ and ____
structure and metabolism
Classification of prokaryotes had been based on ___ and it has now been aided by the use of ____
physical characteristics
DNA analysis
A vast number of prokaryotes have not been studied because
they cannot be cultured
Most prokaryotes exhibit one of the three basic shapes
bacilli, cocci, and spirals
one or several of these structures may be found on the outer surface of many prokaryotes, used to propel the organisms in a fluid environment
Flagella