Chapter 20 Flashcards

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1
Q

Gene

A

A segment of DNA that codes for a protein

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2
Q

Double Helix

A

A twisted ladder or twisted ribbon

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3
Q

DNA

A

Deoxyribonucleic acid

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4
Q

DNA Replication

A

DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule. Half the old DNA, half the new

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5
Q

DNA Helicase

A

DNA helicases are essential during DNA replication because they separate double-stranded DNA into single strands allowing each strand to be copied. They do this by breaking down the H-bonds between complementary base pairs

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6
Q

DNA ligase

A

Glues together the sugar-phosphate backbone and DNA fragments

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7
Q

DNA Polymerase 1 & 3

A

An enzyme that acts as a “proof-reader”

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8
Q

Steps of DNA separation

A
  1. DNA helicase unwinds the helix by breaking down the H-bonds between base pairs
  2. DNA Polymerase 3 links together the free nucleotides
  3. DNA Ligase glues together the sugar-phosphate backbone and DNA fragments
  4. DNA Polymerase 1&3 fill gaps that arise during replication, repair and recombination. “Proof-readers”
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9
Q

RNA

A

RNA carries genetic information that is translated by ribosomes into various proteins necessary for cellular processes

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10
Q

RNA vs. DNA

A

RNA contains ribose sugar instead of DNA’s deoxyribose sugar. RNA has Uracil instead of Thymine. RNA is single stranded, DNA is double stranded. DNA stays in the nucleus, RNA travels as a messenger

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11
Q

Protein Synthesis Steps

A
  1. Transcription (in nucleus)
  2. Translation (in cytoplasm)
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12
Q

Transcription

A

the process of making an RNA copy of a gene’s DNA sequence. This copy, called messenger RNA (mRNA), carries the gene’s protein information encoded in DNA.

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13
Q

Nucleotides

A
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14
Q

Replication Fork

A

a Y-shaped region where the parent DNA double helix splits into two strands, subsequently copied

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15
Q

Translation

A

Translation takes place on ribosomes in the cell cytoplasm, where mRNA is read and translated into the string of amino acid chains that make up the synthesized protein.

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16
Q

DNA sequencing

A

DNA sequencing is the process of determining the sequence of nucleotides (As, Ts, Cs, and Gs) in a piece of DNA

17
Q

Gel electrophoresis

A

Gel electrophoresis is a laboratory method used to separate mixtures of DNA, RNA, or proteins according to molecular size

18
Q

Bacterial Plasmids

A

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell’s chromosomal DNA.