Chapter 2: The Molecular Nature of Genes Flashcards by Cody Coblentz

Q

temperature
DNA conc
pH
time

A

renaturation variables (x4)

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Q

DNA nucleotide components

A

nitrogenous base, sugar, phosphate group

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Q

join polynucleotide strands of DNA and RNA

A

phosphodiester bonds

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Q

(renaturation)=process of bringing together 2 complementary nucleotide strands

A

Annealing (aka?)

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Q

hybridization

A

process of bringing together a complimentary strand of DNA and RNA which occurs at higher temp than DNA/DNA annealing

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Q

transformation with Strept pneumoniae

A

frederick griffith

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Q

phage proteins remained outside bacterial cells while phage DNA entered cells

isotopically labelled T2 protein with S (red) and DNA with P (orange), mixed T2 with E. coli

A

alfred hershey & martha chase experiment

experiment?

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Q

nitrogenous base, sugar, phosphate group

A

DNA nucleotide components

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Q

bases joined together in DNA & RNA

e.g.: adenine base=adenosine in RNA, deoxyadenosine in DNA

A

nucleoside units (e.g.)

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Q

subunits of DNA and RNA

nucleosides w/ phosphate group

A

nucleotides

composed of?

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Q

hydrophobic, 10 bases per turn

A

Bases: property and number

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Q

5’ vs 3’

A

5’=free phosphate, 3’=hydroxyl group

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Q

Smooth Strept. pneumoniae pathogenicity

A

contain capsules protecting them from animal’s defense system

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Q

phosphodiester bonds

A

join polynucleotide strands of DNA and RNA

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Q

Transformation

A

a change in genotype and phenotype due to the assimilation of external DNA by a cell

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Q

renaturation variables (x4)

A

temperature
DNA conc
pH
time

Q

Bases: property and number

A

hydrophobic, 10 bases per turn

Q

alfred hershey & martha chase experiment

experiment?

A

phage proteins remained outside bacterial cells while phage DNA entered cells

isotopically labelled T2 protein with S (red) and DNA with P (orange), mixed T2 with E. coli

Q

nucleoside units (e.g.)

A

bases joined together in DNA & RNA

e.g.: adenine base=adenosine in RNA, deoxyadenosine in DNA

Q

process of bringing together a complimentary strand of DNA and RNA which occurs at higher temp than DNA/DNA annealing

A

hybridization

Q

found that DNA base composition varies between species

DNA/RNA composed of purine and pyrimidine bases: adenine & guanine; cytosine, uracil (RNA), & thymine

A

erwin chargaff (x2)

Q

nucleotides

composed of?

A

subunits of DNA and RNA

nucleosides w/ phosphate group

Q

a change in genotype and phenotype due to the assimilation of external DNA by a cell

A

Transormation

Q

5’=free phosphate, 3’=hydroxyl group

A

5’ vs 3’

Q

frederick griffith

A

transformation with Strept pneumoniae

Q

Annealing (aka?)

A

(renaturation)=process of bringing together 2 complementary nucleotide strands

Q

contain capsules protecting them from animal’s defense system

A

Smooth Strept. pneumoniae pathogenicity

Q

erwin chargaff (x2)

A

found that DNA base composition varies between species

DNA/RNA composed of purine and pyrimidine bases: adenine & guanine, cytosine, uracil (RNA), and thymine