Chapter 2: The Molecular Nature of Genes Flashcards
- temperature
- DNA conc
- pH
- time
renaturation variables (x4)
DNA nucleotide components
nitrogenous base, sugar, phosphate group
join polynucleotide strands of DNA and RNA
phosphodiester bonds
(renaturation)=process of bringing together 2 complementary nucleotide strands
Annealing (aka?)
hybridization
process of bringing together a complimentary strand of DNA and RNA which occurs at higher temp than DNA/DNA annealing
transformation with Strept pneumoniae
frederick griffith
phage proteins remained outside bacterial cells while phage DNA entered cells
isotopically labelled T2 protein with S (red) and DNA with P (orange), mixed T2 with E. coli
alfred hershey & martha chase experiment
experiment?
nitrogenous base, sugar, phosphate group
DNA nucleotide components
bases joined together in DNA & RNA
e.g.: adenine base=adenosine in RNA, deoxyadenosine in DNA
nucleoside units (e.g.)
subunits of DNA and RNA
nucleosides w/ phosphate group
nucleotides
composed of?
hydrophobic, 10 bases per turn
Bases: property and number
5’ vs 3’
5’=free phosphate, 3’=hydroxyl group
Smooth Strept. pneumoniae pathogenicity
contain capsules protecting them from animal’s defense system
phosphodiester bonds
join polynucleotide strands of DNA and RNA
Transformation
a change in genotype and phenotype due to the assimilation of external DNA by a cell