Chapter 2- microbial Cell Structure And Fuction Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Different types of light microscopy

A

Bright field
Phase contrast
Dark Field
Fluorescent

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Specimen are visualized because of differences in constrats of specimen and environment

A

Bright field

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Magnification

A

Ability to make object larger

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Resolution

A

Ability to distinguish two adjacent object as separate and disticnt

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Dyes

A

Organic compounds that bind to specific cellular materials

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Example of common stains

A

Methylene blue
Crystal violet
Safranin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Chromphore

A

Coloured portion of dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

One dye used to color specimen

A

Simple staining

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Two type of dye:

A

Basic dye

Acidic dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Positively charged chromophore. Binds to negatively charged cellular materials

A

Basic dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Acidic dye

A

Negatively charged chromophore repelled by cell surface result to background staining or negative stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Difference of gram positive and gram negative. What colour they appear.

A

Gram positive, thicker peptidoglycan
Purple

Gram negative. Thin peptidoglycan
Pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Example of differential stain

A

Gram stain
Acid fast
Endospore stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Acid fast stain and what color of stains. Primary and secondary

A

Detects mycolic avid in cell wall of mycobacterium
Primary stain. Pink
Other blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Endospore stain colour primary and counterstain

A

Primary green

Counter pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Phase contrast

A

Amplifies differences in REFRACTIVE INDEX of cell and environment.

No dye necessary
For live sample

17
Q

How do samples appear in phase contrast?

A

Sample is dark

Light BG

18
Q

Darkfield microscopy and how does specimen appear?

A

Specimen is illuminated with hollow cone of light. Refracted light enter obj

Bright specimen
Dark BG

19
Q

Use to visualize specimens that fluoresce. Emit light color when illuminated with another color of light

A

Fluorescence microscopy

20
Q

Imaging cells in 3D and define

A

Differential interference contrast microscopy- uses polarizer to create beams of polarized light
Confocal scanning laser microscopy- laser source to generate 3d image

21
Q

Microscope for cell structure

A

Transmission electron microscope - through internal structure
Scanning electron microscope- surface

22
Q

TEM must be stained with _____ while SEM must be coated with _____

A

Metals lead or uranium

Gold

23
Q

Cell shape
Sphere
Rids
Spirals

A

Cocci
Bacilli
Spirochetes, spirillum

24
Q

Structure that surrounds the cell and a barrier that separates cytoplasm from envr

A

Cytoplasmic membrane

25
Q

Bacteria and eukarya contains _____ linkage, archaea contaims ___ linkage

A

Esther, ether

26
Q

Proteins in membranse. Differentiate integral with perioheral

A

Integral - embedded

Peripheral- surface

27
Q

Nutrient transport events

A

Uniporter
Symporter
Antiporter

28
Q

Three major classes of transport systems in prokaryotes and give ex

A

Simple transport- lac permease of e. Coli
Group translocation- phosphotransferase
ABC system- ato binding cassettes

29
Q

Compound microscope

A

Uses visible light to illuminate cells