Chapter 18: Methods in Microbial Ecology Flashcards
1
Q
Roles of microbes in ecosystems
A
Production of particulate carbon
Decomposition
Nutrient cycling
Interactions with other organisms
2
Q
Reason for difficulty culturing all microbes
A
Competition from faster growing microbes
Not used to working with scale
3
Q
Culture-dependent methods of analysis
A
Growth on media
Winogradsky column
Spectrophotometry
4
Q
Winogradsky column
A
Artificial ecosystem
sulfur rich soil in tube with foil cap at top
High O2 concentration at top, high H2s concentration at bottom
5
Q
Drawback of culture-dependent analyses
A
Not true measures of abundance, only isolation of certain metabolic types
6
Q
Culture-independent methods of analysis
A
Microscopy
Flow cytometry
Genetic analysis
7
Q
“semi-dependent” measures of microbe activity
A
chemical measures
isotopic tracers
