CHAPTER 15: AUTOMATED BLOOD CELL ANALYSIS Flashcards
Refer to: https://drive.google.com/file/d/1bcK7U5-tZkTLCncYZ8umFXzSoUI7wqZr/view
(1) Which printout lets the end user know at a glance that the results are acceptable and no manual work needs to be performed?
a. CELL-DYN Sapphire
b. UniCel DxH 800
c. ADVIA 2120i
d. Sysmex XN-series
d. Sysmex XN-series
Refer to: https://drive.google.com/file/d/1bcK7U5-tZkTLCncYZ8umFXzSoUI7wqZr/view
(2) Which instrument printout has a system flag on the platelet count?
a. CELL-DYN Sapphire
b. UniCel DxH 800
c. ADVIA 2120i
d. XN-series
a. CELL-DYN Sapphire
Refer to: https://drive.google.com/file/d/1bcK7U5-tZkTLCncYZ8umFXzSoUI7wqZr/view
(3) What do you suspect is the cause of the variation in platelet counting among the four instruments?
a. Different instruments have different levels of sensitivity.
b. All instruments use the same principle for counting platelets.
c. Some instruments are susceptible to false-positive platelet flagging under certain conditions.
d. Different instruments use different thresholds to capture and count platelets.
d. Different instruments use different thresholds to capture and count platelets.
Refer to: https://drive.google.com/file/d/1bcK7U5-tZkTLCncYZ8umFXzSoUI7wqZr/view
(4) Based on the overall flagging for this specimen on each instrument, should a manual differential count be performed for this patient?
a. Yes, because immature granulocytes are present in the sample.
b. Yes, because the WBC scatterplots are abnormal.
c. No, because each differential count is complete with no system or morphology flags.
c. No, because each differential count is complete with no system or morphology flags.
(5) A patient peripheral blood film demonstrates agglutinated RBCs, and the CBC shows an elevated MCHC. What other parameters will be affected by the agglutination of the RBCs?
a. MCV will be decreased and the RBC count will be increased.
b. MCV will be decreased and the RBC count will be decreased.
c. MCV will be increased and the RBC count will be decreased.
d. MCV will be increased and the RBC count will be increased
c. MCV will be increased and the RBC count will be decreased.
(6a) Match the cell-counting method listed with the appropriate definition:
IMPEDANCE
a. Uses diffraction, reflection, and refraction of light waves
b. Uses high-voltage electrical waves to measure the internal complexity of cells
c. Involves detection and measurement of changes in electrical current between two electrodes
c. Involves detection and measurement of changes in electrical current between two electrodes
(6b) Match the cell-counting method listed with the appropriate definition:
RF
a. Uses diffraction, reflection, and refraction of light waves
b. Uses high-voltage electrical waves to measure the internal complexity of cells
c. Involves detection and measurement of changes in electrical current between two electrodes
b. Uses high-voltage electrical waves to measure the internal complexity of cells
(6c) Match the cell-counting method listed with the appropriate definition:
OPTICAL SCATTER
a. Uses diffraction, reflection, and refraction of light waves
b. Uses high-voltage electrical waves to measure the internal complexity of cells
c. Involves detection and measurement of changes in electrical current between two electrodes
a. Uses diffraction, reflection, and refraction of light waves
(7) Low-voltage DC is used to measure:
a. Cell nuclear volume
b. Total cell volume
c. Cellular complexity in the nucleus
d. Cellular complexity in the cytoplasm
b. Total cell volume
(8) Orthogonal light scatter is used to measure:
a. Cell volume
b. Internal complexity of the cell
c. Cellular granularity
d. Nuclear density
b. Internal complexity of the cell
(9) On the Beckman Coulter instruments, hematocrit is a calculated value. Which of the following directly measured parameters is used in the calculation of this value?
a. RDW
b. Hemoglobin
c. MCV
d. MCHC
c. MCV
(10a) Match the instrument listed with the technology it uses to determine WBC differential counts.
ABBOTT CELL-DYN SAPPHIRE
a. Volume, conductivity, and five angles of light scatter
b. MAPSS technology and three-color fluorescence
c. Peroxidase-staining absorbance and light scatter
d. Detection of forward and side scattered light and fluorescence
b. MAPSS technology and three-color fluorescence
(10b) Match the instrument listed with the technology it uses to determine WBC differential counts.
SIEMENS ADVIA 2120i
a. Volume, conductivity, and five angles of light scatter
b. MAPSS technology and three-color fluorescence
c. Peroxidase-staining absorbance and light scatter
d. Detection of forward and side scattered light and fluorescence
c. Peroxidase-staining absorbance and light scatter
(10c) Match the instrument listed with the technology it uses to determine WBC differential counts.
SYSMEX XN-1000
a. Volume, conductivity, and five angles of light scatter
b. MAPSS technology and three-color fluorescence
c. Peroxidase-staining absorbance and light scatter
d. Detection of forward and side scattered light and fluorescence
d. Detection of forward and side scattered light and fluorescence
(10d) Match the instrument listed with the technology it uses to determine WBC differential counts.
BECKMAN COULTER UNICEL DxH 800
a. Volume, conductivity, and five angles of light scatter
b. MAPSS technology and three-color fluorescence
c. Peroxidase-staining absorbance and light scatter
d. Detection of forward and side scattered light and fluorescence
a. Volume, conductivity, and five angles of light scatter
