Chapter 14 - Energy Generation in Mitochondria and Chloroplasts Flashcards
Proton gradient flow down their electrochemical gradient through _____.
ATP synthase
Mitochondria produce most of the animal cells’ ATP using energy derived from ______.
Oxidation of sugars and fatty acids.
The 3 stages of cellular respiration are
1- Glycolysis 2- Citric acid cycle 3- Oxidative phosphorylation
What breaks down glucose into two molecules of pyruvate?
Glycolysis
What completes the breakdown of glucose?
Citric acid cycle
What accounts for most of the ATP synthesis?
Oxidative phosphorylation
Each pyruvate has __ carbons.
3
In glycolysis, 6 carbon glucose is broken into
two 3-carbon pyruvate
In cellular respiration…. When oxygen is available, pyruvate enter the citric acid cycle. But first, they must convert to _____ first.
acetyl CoA
Where does glycolysis occur?
cytosol
Where does the citric acid cycle occur?
Mitochondria matrix
Where does oxidative phosphorylation occur?
inner membrane of mitochondria
Where does Pyruvate (3C) convert to Acetyl CoA (2C)?
mitochondria
_____ accounts for almost 90% of the ATP generated by cellular respiration.
oxidative phosphorylation
A smaller amount of ATP is formed in glycolysis and citric acid cycle by _____.
substrate level phosphorylation
For each molecule of glucose degraded to CO2 and water by respiration, the cell makes up to ___ molecules of ATP.
32
What section of the mitochondria contains porin which forms large channels?
outer membrane
NADH and FADH2 are _______ generated during ____ to power the production of ATP.
activated carriers; citric acid cycle
Okay so mitochondria produce lots of ATP by ___________.
oxidative phosphorylation.
What describes the phosphorylation of ADP to create ATP using energy derived from the transfer of electrons to oxygen?
oxidative phosphorylation
What process describes the pH gradient that drives an energy requiring process?
chemiosmotic coupling
In stage 1 of the oxidative phosphorylation, ___ is produced.
proton gradient
in stage 2 of the oxidative phosphorylation, the proton gradient produced in stage 1 is used to drive a variety pf energy requiring reactions including the synthesis of ___ by an ___ synthase.
ATP; ATP
The electron transport chain is composed of 3 ________ complex.
integral membrane
_____ catalyze that reduction of oxygen to water.
cytochrome c oxidase
Which of the components of the ETC is required to combine the pair of electrons with molecular oxygen?
cytochrome c oxidase
The pH in the mitochondrial matrix is higher than the pH in the intermembrane space. True or false?
True
What is the first complex in the ETC.
NADH dehydrogenase complex
How do you know electrons flow from complex one to two to three?
Electron movement is redox reaction.
What measures the tendency of a molecule to be reduced by taking up new electrons?
Redox potential
Molecule with the lowest redox potential is ___ complex.
NADH dehydrogenase complex
Molecule with the greatest redox potential is ___ complex.
cytochrome c oxidase complex
Molecules with a ____ redox potential are more likely to give up their electrons, and thus have a ____affinity for electrons.
low; low
Electron Transport Chain is energetically unfavorable because the electrons are passed from the complex that has a lower electron affinity to one with the higher electron affinity. True or false?
False. It’s energetically favorable.
Electron Transport Chain is energetically favorable because the electrons are passed from the complex that has a lower electron affinity to one with the higher electron affinity. True or false?
True
Proton pumping generate pH gradient and membrane potential. True or false?
True
Stage 1 of oxidative phosphorylation requires the movement of electrons along the electron-transport chain coupled to the pumping of protons into the inter-membrane space. What is the final result of these electron transfers?
O2 is reduces to H20
Which of the following terms describes ATP synthesis in mitochondria?
oxidative phosphorylation
____ is a small hydrophobic molecule that diffuses within the lipid bi-layer.
quinone
Quinone is an electron carrier type. It is a small hydrophobic molecule that diffuses within the lipid bi-layer. And it accepts 1 or 2 electrons from ___ or ____.
NADH or FADH2
_____ is an electron carrier type that contain iron and sulfur molecules and are covalently attached to ______. It holds ___ electron(s).
Iron-sulfur centers; iron-sulfur proteins; 1
The third type of electron carrier is the _______ that contain iron molecules and are covalently attached to ____ proteins. It holds __ electron(s).
Heme groups; cytochrome; 1
During reduction and oxidation, _____ pick up an H+ on one side of the membrane and release it on the other.
electron carriers
What are the two advantages of the components of the ETC being embedded in the inner mitochondrial membrane? Give an example too.
1) The first one is components in the proper orientation for the electron so they can move faster from one component to the next; 2) Components are more organized and faster and have the proper position to facilitate transport proton matrix to the inter-membrane space. Example: ubiquinone
ATP Synthase uses the energy stored in the ______ to produce ATP.
electrochemical proton gradient.
ATP synthase is a _______ coupling device.
reversible
The proton flow through the transmembrane H+ carrier of ATP synthase results in….
Mechanical rotation that is converted into the chemical-bond energy of ATP.
In eukaryotic cells, the electrochemical proton gradient is used to drive what two things?
1- The formation of ATP; 2- The transport of selected metabolites across the inner mitochondrial membrane
Oxidation of one glucose molecule produces about __ ATP molecules.
30 to 32
What is the direct product of glycolysis, and the final ATP yield per molecule of glucose?
2 NADH (cystolic) —> 3 ATP 2 ATP ——————-> 2 ATP
What is the direct product of pyruvate oxidation to acetyl CoA (two per glucose), and the final ATP yield per molecule of glucose?
2 NADH (mitochondrial matrix) —–> 5 ATP
What is the direct product of complete acetyl CoA oxidation (2 per glucose), and the final ATP yield per molecule of glucose?
6 NADH (mitochondrial matrix) ———————–> 15 ATP 2 FADH2 ———> 3 ATP 2 GTP ————–> 2 ATP
Modern eukaryotes depend on mitochondria to generate most of the cell’s ATP. How many molecules of ATP can a single molecule of glucose generate?
30
Photosynthesis is a process that takes place in ____ and uses ___ energy to generate ______ electrons, which are passed along an electron-transport chain.
chloroplasts; light energy; high-energy