Chapter 14 Flashcards

1
Q

Define translation

A

Use of mRNA as instructions to make a protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What 4 things does translation (eukaryotic) use?

A

mRNA, ribosomes, amino acids, and tRNAs.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Define ribosome

A

Machine that reads mRNA and combines amino acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Define amino acids

A

Monomers of proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What does mature eukaryotic mRNA contain (7 things)

A

5’ cap
5’ untranslated region (5’ UTR)
Start codon
Open reading frame
Stop codon
3’ untranslated region (3’ UTR)
Poly-A tail

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does mature prokaryotic mRNA contain (6 things)

A

5’ UTR
Shine-Delgarno Sequence
Start codon
Open reading frame
Stop codon
Poly-A tail

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What does 5’ cap do?

A

Protection and translation initiation in eukaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What does 5’ untranslated region do?

A

Helps control initation of translation in both eukaryotes and prokaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What does a 3’ UTR do?

A

Helps control the amount of translation in eukaryotes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What does the poly-A tail do?

A

Proides stability and protection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the differences in prokaryotes and eukaryotes mRNA?

A

Prokaryotes do NOT have a 5’ cap
Prokaryotes contain a Shine-Delgarno sequence and additional ORFs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Define small subunit

A

Helps initiation of translation by finding and binding to the start codon
Made up of multiple proteins and RNA molecules.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Define the large subunit

A

accepts tRNAs and makes polypeptides.
Made up of multiple proteins and RNA molecules.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Define a dalton

A

The mass of 1 Hydrogen. Used to measure protein size.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the 3 main components of the large subunit and what do they do?

A
  • Acceptor (A) Site - new tRNAs bind here
  • Peptide (P) Site - Polypeptide elongates here
  • Exit (E) Site - Old tRNA leaves from here
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What does rRNA do?

A

In the large subunit, the rRNA catalyzes polypeptide formation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Define ribozymes

A

rRNA molecule that catalyzes a reaction

18
Q

How many amino acids are there? What are the unconventional amino acids?

A

20 conventional.
2 unconventional: selenocysteine in humans, and pyrrolysine in bacteria.

19
Q

What do all amino acids contain?

A

An amino group, carboxyl group, central carbon, and a chemically distinct side chain (R-group).

20
Q

How does the ribosome link multiple amino acids together?

A

Peptide bond, with Carboxyl group being attached to the amino group of the next.

20
Q

Define N terminus

A

Front end of polypeptide - the exposed amino group

21
Q

Define C terminus

A

Back end of polypeptide - exposed carboxyl group

22
Q

Like a DNA strand going 5’ -> 3’, the polypeptide goes ….

A

N terminus -> C terminus

23
Q

Define tRNA

A

Transfer RNA reads the codon and brings the corresponding amino acid.

24
Q

Define anticodon

A

Region complementary to the codon

25
Q

Define acceptor stem

A

Region that brings the amino acid

26
Q

How many different tRNAs are there?

A

64 - each codon needs a different tRNA

27
Q

Define tRNA charging

A

Chemical reaction that first attaches amino acids to tRNA. The tRNA then take the amino acid to the ribosome

28
Q

Define aminoacyl tRNA synthetases

A

Enzymes that catalyze the attachment of the amino acid to the tRNA.
Each amino acid has a different synthetase.

30
Q

What are the steps to prokaryotic translation initation?

A
  1. Small ribosomal subunit binds to the Shine-delgarno sequence
  2. Initation factors (IFs) bring methionine tRNA to the start codon
  3. Large ribosomal subunit joins and IF’s leave
31
Q

Where is the shine-delgarno sequence?

A

~6 nucleotides ahead of the AUG start codon

32
Q

What are the steps of elongation in prokaryotes?

A
  1. A new, charged tRNA enters ribosome A site. The elongation factor EF-Tu carries the tRNA in.
  2. Ribosome shifts forward and makes a peptide bond between the amino acids in the A and P sites.
  3. Old, now uncharged, tRNA leaves the E site as a new one enters the A site
33
Q

What is EF-Tu

A

Elongation factor in prokaryotes that carries tRNA into the A site

34
Q

What are the steps for translation termination in prokaryotes?

A
  1. Ribosome reaches stop codon
  2. Release factor enters the A site. Causes dismantling of the complex.
35
Q

What are the 3 main differences between eukaryotic and prokaryotic translation?

A
  • Eukayotes have a 5’ cap instead of a shine-delgarno sequence to help small subunit bind to the mRNA.
  • Eukaryotes have more initiation factors - more regulation
  • mRNA may form a circle in Eukaryotes allowing for translation to quickly repeat.
36
Q

Define polysomes

A

When multiple ribosomes attach to the mRNA, allowing for multiple translations to occur. This allows for many proteins to be produced quickly.

37
Q

What 3 steps do new proteins undergo?

A
  1. Folding
  2. Protein targeting
  3. Post-translational modifications
38
Q

Define protein folding

A

Adoption of a 3D structure based on its amino acid sequence

39
Q

Define protein targeting

A

Being taken to its proper location in the cell, based on instructions embedded in its sequence.

40
Q

Define post-translational modifications

A

Individual amino acids get chemically altered, changing the function of the protein.