Chapter 11 Microbial World And You Flashcards
How are the domains and kingdoms now classified?
Via rRNA signatures
Peptidoglycan rRNA signatures gives rise to what Domain?
Pseudomurein rRNA signature gives rise to what Domain?
Domain Bacteria
Domain Archaea
Domain Bacteria is broken down by what distinction
Gram - and Gram +
Gram negative Bacteria is further broken down into what two groups?
Proteobacteria and Nonproteobacteria
How is Gram positive bacteria broken down by?
Low G+C nucleotides and High G+C nucleotides
In Domain Bacteria, how did Proteobacteria get its name?
What are its Characteristics?
From the mythical Greek god Proteus, who could assume MANY SHAPES
Gram Negative
Chemoheterotrophic
They have a photosynthetic ancestor
The relationship is based on nucleotide sequence of ribosomal RNA
How are the subgroups designated by?
Give the names?
Subgroups are designated by Greek letters Alphaproteobacteria Betaproteobacteria Gammaproteobacteria Deltaproteobacteria Epsilonproteobacteria
What are the characteristics of alpha bacteria?
What is the most abundant living organism on earth and where is it found?
Capable of growth at low level nutrients
Some with unusual morphology such as: prosthecae- stalks or buds
Pelagibacter ubique: the most abundant living organism on earth in the ocean
They are very small; only 1354 genes
What are the human pathogens?
Bartonella
Brucellosis
Ehrilichia
Rickettsia
What is Bartonella?
-B. henselae: cat scratch disease
What is Brucella?
What does it cause and its key features?
causes Brucellosis or Malta Fever
Fluctuating fever that spikes every afternoon that is why it is called Undulant Fever
Survives phagocytosis
Ingestion of contaminated milk
What is Ehrilichia? What are its characteristics and how is it transmitted?
Transmitted by ticks
Causes the disease Ehrilichiosis (fever, chills, nausea)
It’s a tick-borne human disease
Obligate Intracellular parasites (WBC)
What is Rickettsia? Characteristics?
Rickettsia: Arthropod-borne. Cause number of diseases know as spotted fever group
What are the three forms of Rickettsia and how are they transmitted?
R. prowazekii - Epidemic Typhus. Transmitted by lice
R. typhi- Ednemic Murine Typhus. Transmitted by the Rat Flea
R. rickettsii - Rocky Mountain spotted fever. Transmitted by tick
For Alphaproteobacteria, they have a prosthecae? What is it and what is it’s purpose?
Have a prosthecae (stalk)
Used for attachment and nutrient absorption
How do alpha reproduce?
No binary fission
Reproduce by bidding (Asexual reproduction)
What Stalked bacteria are found in lakes?
Caulobacter
What budding bacteria are found in lakes? Where else do these grow?
Hyphomicrobium
They also grow in lab water baths
What is Agrobacteriaum?
What is type of research is it important in?
Plant pathogen
They insert a plasma into plant cells, inducing a tumor called a Crown Gall
Important in Biotechnology
Alphaproteobacteria use what for energy? How do they do this?
They are Chemoautotophic and oxidize nitrogen for energy
They fix CO2
How is nitrogen oxidized for Nitrosomonas and Nitrobacter?
Nitrosomonas: NH3 (ammonia).–> NO2- (nitrite)
Nitrobacter: NO2- ( nitrite) —> NO,- (nitrate; a better nitrogen source for plants
What are these nitrogen using bacteria called and what are they important for?
Nitrifying Bacteria
They are important for agriculture
What does Azosprillum and Rhizobium have in common?
Both are nitrogen fixing bacteria
What are the characteristics of Azospirillum? Are they aerobic or anaerobic?
Anaerobes
Lives in close association with the roots of plants (beans legumes)
Nitrogen fixation
What are the characteristics of Rhizobium? Where do they do nitrogen fixation?
Fix nitrogen in the roots of plants (symbiosis)
They induce nodules so important in agriculture by infects the roots of plants beans legumes clovers
What is Acetobacter and Gluconobacter? What is their significance?
What does this produce in industry?
What can this destroy?
Produce acetic acid form ethanol, so important to industry
They are aerobic
Ethanol to Acetic acid (vinegar)
This spoils wine
What are the general characteristics of Betaproteobacteria?
Use Hydrogen gas, ammonia and methane
Some are pathogens
It overlaps with alpha
What is Thiobacillus (Acidithiobacillus)?
What do they oxidize (from what to what)?
Sulfur-oxidizing bacteria: Chemoautotrophic
The obtain energy by oxidizing:
H2S or S (elemental sulfur). To SO42- (sulfate)
What is Spirillum?
What type of energy source and carbon sources does Spirillum use?
Where are they found
Chemoheterotrophic, helical
In fresh water
They are Gram negative
How are Spirillum and Spirochetes different?
Spirillum are motile by a polar flagella
What is Sphaerotilus natans?
Energy, and carbon source type?
Where are they found
Chemoheterotrophic; that form sheaths
Found in fresh water and sewage
Gram negative
What does Sphaerotilus natans have for protection?
What is one if the problems that this contributes to ?
Sheath is for protection and nutrient accumulation
Contributes to bilking; a problem in sewage treatment
What is Neisseria?
Energy and carbon source?
Where are they commonly found?
Chemoheterotrophic; aerobic cocci
Gram negative
Normal Microbiota of the mucous membrane
What are the pathogenic species of Neisseria?
What do they cause?
N.meningitidis: causes meningitis
N.gonorrhoea: causes gonorrhoea
What is Bordetella?
What does it cause?
Energy and carbon source?
Chemoheterotrophic, gram negative, non motile, rods
B.pertussis: causes whooping cough
What is Burkholderia?
What does it cause and where is it primarily found?
Is it motile and if so how?
All motile with polar flagella or tuft.
Gram -
Nosocomial (hospital) infections or Healthcare-associated infections (HAI)
What is the best know species of Burkholderia and where does it grow?
What is this a problem in specific patients? What does it do to their respiratory tract?
B.cepacia: can grow in disinfectants, can degrade more than 100 different organic molecules ad contaminate hospital equipment and drugs
Problem in cystic fibrosis patients because it metabolizes accumulated respiratory secretions
What HAI’s are the greatest since 2011
Pneumonia
Surgical site infection from any inpatient surgery
Gastrointestinal illness
In 2014 there was a HAI study published, what was the results?
2011- there were ~ 722,000 HAI’s in US acute care hospitals
And additionally, about 75,000 patients with HAI’s dies during their hospitalization.
What is the Betaproteobacteria Zoogloea?
Important in sewage-treatment processes such as the activated sludge system.
They form fluffy, slimy mass which is important in the activated sludge system
What is the largest subgroup of Proteobacteria?
Gammaproteobacteria
What are Pseudomonadales? Pseudomonas?
Are the motile and how?
Gram - aerobic rods or cocci
Rods are motile with polar flagella either single or tuft
Opportunistic pathogens in immunocompromised patients
Describe Pseudomonas aeruginosa?
What does it cause
Produces a soluble blue-pigmentation
In weakened patients it can cause: urinary tract infections, but, wound, septicemia, abscesses and meningitis.
Where can P.aeruginosa grow,a new what type of respiration doe they perform?
where are they found?
Can grow in the fridge
Although aerobic, can also upset nitrate to perform anaerobic respiration meaning use nitrogen and final electron acceptor (loss of nitrogen in fertilizer)
They are found in soil and water
They can decompose pesticides that are added to soil
What is Moraxella lacunata?
What does it cause?
Aerobic coccobacilli
Cause Conjunctivitis: inflammation of the eye membrane
What are Azotobacter and Azomonas?
What is their primary function?
Nitrogen fixing
Free living in the soil and important in agriculture
What is Legionella?
Where is it found and what does it cause?
Found in Streams, warm-water pipes, cooling towers.
Intracellular parasites
Causes L.pneumophila (Leginnaires’ disease)
Outbreak in NY 2015
What is Coxiella burnetii?
What does it cause and how is it transmitted?
What is now done to stop this?
Causes Q fever and transmitted by contaminated milk from cattle ticks that harbor the organism.
That is why milk is pasteurized
It’s and intracellular parasite
May form endospores
What is Gammaproteobacteria Vibrio?
What type of respiration does it do?
Where is it found
Anaerobic Gram negative curved rods that are found in mostly aquatic habitats - coastal water
What are two species that are pathogenic of Vibrio?
What do they cause?
V.cholerae causes cholera
V.parahaemolyticus: causes gastroenteritis from raw undercooked shellfish
What is Enteronacteriaceae? What else are they called
Where are they found?
Facultative anaerobic gram negative rods
Also called enterics or coliform
They inhabit the intestinal tract of humans and animals.
How do Enterobacteriaceae move?
They are Peritrichous flagella
What are the characteristics of Enterobacteriacea?
Have fimbriae which help them adhere to surface or mucous membrane
Have sex pili to exchange genetic material which often included antibiotic resistance
Ferment glucose and other carbs
What are some of the important genera of Enterobacteriaceae?
Enterobacter Erwinia Escherichia Klebsiella Proteus Salmonella Serratia Shigella Yersinia
What is Escherichia coli? What are its characteristics?
Produces bacteriocin: lyses closely related bacteria. It’s a facultative anaerobe that inhabits the human intestinal tract. (Which this form is not a pathogen)
Some produce toxins E.coli O157:H7
What can E.coli cause?
Could cause urinary infections and traveler’s diarrhea (which is a food-borne disease)
What is Samonella and what are its characteristics?
Where is it found?
All are pathogens
Found in the normal Microbiota of intestinal tract of poultry and cattle which are warm blooded animals
What species which is pathogenic is divided into more art 2400 serovars?
Salmonella enterica
What does Serotype mean?
What is a Serovars of S. enterica?
Serovars or serotypes are serological varieties that is often used to mean the same thing
S.typhimurium is a serovars of S.enterica
What it’s the Kauffman-White scheme and what does it depend on?
The Kauffman-White scheme is serotyping and it depends on antigens (Ag) on
K capsule
O cell wall
H flagella
What does S.typhi cause?
Causes typhoid fever (the most sever)