Chapter 11- Chromosomes Flashcards
The level of DNA structure that is the nucleotide sequence
Primary Structure
The level of DNA structure that is the double-stranded helix
Secondary structure
The level of DNA structure that is the high folding of DNA that takes place in cells
Tertiary structure
Additional winding/twisting of the double-helix DNA
Supercoiling
Clockwise supercoiling, overrotated
Positive supercoiling
Counterclockwise supercoiling, underrotated
Negative supercoiling
Proteins that add or remove rotations in DNA by breaking and then rejoining DNA strands
Topoisomerase
The type of topoisomerase that adds makes single strand breaks
Type I
The type of topoisomerase that makes double stranded breaks
Type II
The prokaryote version of a nucleus, where the DNA is
Nucleoid
The DNA and associated proteins of a chromosome in eukaryotes
Chromatin
Proteins in chromatin (eukaryotes) that play a structural role, roles in chromosome replication, chromosome segregation, and transcription
Non-histone Proteins
Small, positively charged, highly conserves proteins found in all eukaryotes, make up half the proteins in chromatin
Histones
The octamer of the four core histones H2A, H2B, H3, and H4
Nucleosome
One nucleosome is present ever how many base pairs
Around 200 bp (145-147 wrapped around the nucleosome, 20 bound to histone H1, and 30-40 bp of linker DNA)
The diameter of chromatin at the nucleosome level
11 nm
The diameter of chromatin when is is coiling in helical arrays, second level
30 nm
The diameter of chromatin in the third level, when it forms large loops
300 nm