Chapter 1: Histology and Its Methods of Study Flashcards
define microtome
instrument to cut very small tissue sections
define fixation
placing a tissue in a solution that preserves the sample
define basophilic
to have affinity for basic components
define eosinophilic
cells appearance that take up the eosin dye
hematozylin & eosin (H&E)
combination of stains used to prepare tissue samples
define immunochemistry
histochemistry technique that uses antibodies to detect specific proteins
define antibody
immunoglobulin family that binds to antigens to eliminate them
what is histology?
the study of tissues
in what disciplines is histology relevant?
- research
- pathological examination
- medical diagnosis
list the steps in preparing a sample for LM
- fixation
- dehydration
- clearing
- infiltration
- embedding
- trimming
describe the fixation step
placing tissue in a solution that cross-links proteins and deactivates enzymes preserving the sample
describe the dehydration step
transferring tissue through varying concentrations of alcohol to remove all water
describe the clearing step
using organic solvents to remove all residing alcohol
describe infiltration step
infiltrating sample with paraffin wax
describe the embedding step
placing sample in a mold w/ melted paraffin
describe the trimming step
trimming the hardened paraffin block to expose the tissue for sectioning
what is the maximum magnification and resolution of LM?
1000x
0.2 micrometers
what is the use of LM?
uses light w/ tissue components to reveal and study tissues
what is the maximum magnification and resolution of TEM?
120,000x
0.1-3 nm
what is the use of TEM?
shows detailed structures of cellular organelles
what is the maximum magnification and resolution of SEM
1,000,000x
0.5 nm
what is the use of SEM?
to view cellular surfaces in 3D
