Chap 14 Flashcards
Genomes
1
Q
Genome
A
All DNA in a HAPLOID set of chromosomes
2
Q
Cytogenetic map
A
- Chromosonal band
- Detect abnormalities
3
Q
Linkage map
A
- Inheritance patterns
- Recombination frequency
4
Q
Physical Map
A
- Location of genes/ markers
5
Q
Sequence Map
A
- DNA sequence
- Base pairs
6
Q
Sequencing the Human Genome
A
- Old technique: clone by clone
- New technique: Whole genone shotgun
7
Q
Whole Genome Sequence (Shotgun)
A
- Shotgunned into small fragments.
- Amplify: create multiple copies
- Fill ATGC
- Reconstruct from overlaps
8
Q
Annotation of Genes
A
Descriptions of what genes do
9
Q
Encoded protein perncetage
A
1.5% of genome
10
Q
Exome Sequencing
A
Targeted coding gene
11
Q
Noncoding-gene in genome Includes
A
- Viral DNA
- Noncoding RNAs
- Introns
- Promoters and other control sequences
- Repeated sequences
12
Q
Viral DNA
A
- Retroviruses (RNA) past infection
- 8% of genome
- Human endogenous retroviruses
13
Q
Noncoding RNAs
A
- tRNA: aminoacids
- rRNA: ribosome
- Long noncoding: Control Gene expression
14
Q
Transposons
A
- Repeat sequence move around genome
15
Q
CRISPR-CAS9 function and structure
A
- Cutting DNA at specific location
- Cas9 protein + Single Guide RNA
- Locate and bind to PAM
- Unwind, Match, and Cut
