CH5: Biohazards

Question 1

Good handwashing and glove maintenance…

Answer 1

*Make sure to put gloves on right before entering or immediately after.
*make a habit of cleaning your gloves followed by washing your hands when leaving the lab

Question 2

Bio Safety Lab Requirements: (7)

Answer 2

1. All tools touched should be disinfected and sterilized
2. all cabinets, fridges, and freezers used for storage should be well labeled and disinfected regularly
3. Samples in a centrifuge should be tightly closed
4. Sample vials with rubber stoppers should be carefully opened to avoid aerosolized contaminated drops
5. Don’t use your mouth for anything
6. Don’t fix your hair, your face, nose, or eyes. Gloves shouldn’t touch your skin.
7. Know where the emergency stations are!!

Question 3

BSL lab classifications

Answer 3

BSL-1: permits work with harmless microbes
BSL-2: works with infectious microbes
BSL-3: microbes known for epidemy with known treatments
BSL-4: microbes known for pandemics (SARS)

Question 4

Biological Safety Cabinet procedures:

Answer 4

*Disinfect before and after using, and before taking anything out
*Place everything you need inside before working
*Allow airflow to start at least 15 mins before and after working
*Only use slow and intentional hand movements when working
*Keep trash can inside BSC
*Disinfect the whole area before leaving the BSC