CH3: The Lab

Question 1

Three Must Have Safety Lab Measures

Answer 1

• Make sure the lab always gives safety procedures particularly about known hazards to that lab
• Know where all safety equipment is and that it’s functional and clean
• All labs should have at least 2 escape paths, make sure these are clean

Question 2

Primary Work Environment

Answer 2

Chemical Resistant - all surfaces should have chemically resistant surfaces (even walls)
Fume hoods - all work with volatile chemicals should be done here
Biohazards - kept in Biological Safety Cabinets (BSC)

Question 3

General Chemical Storage Protocol

Answer 3

Chemicals should be stored in a secondary container, with minimal spill hazards, and correctly and clearly labeled

Question 4

What can Acids be stored with?
What is a no go?

Answer 4

• Acids are separated into organic and oxidizing acids and placed together
• Acids should never be put with bases, alkalis, flammables, active metals, and chemicals that generate toxic gases (e.g. sodium)

Question 5

What can Bases be stored with?
What’s a no go?

Answer 5

• Bases and alkalis are stored together
• Never store acids or flammables with bases, and certain oxidizers and toxic chemicals should be checked before storing together

Question 6

What can Flammables be stored with?
What’s a no go?

Answer 6

*Keep other flammable chemicals together
* Keep flammables away from ignition sources, hot wires, chlorinated solvents, flammable solvents, oxidizing acids, oxidizers, gas pipes, and at a max quantity of 50L

Question 7

What can Oxidizers be stored with?
What’s a no go?

Answer 7

• Oxidizers can be stored together in a cool, dry place
• Oxidizers should be kept away from organic solvents, combustible materials, reducing agents, flammables, and wooden shelves

Question 8

What can Water reactive chemicals be stored with?
What’s a no go?

Answer 8

• Water Reactive Chemicals can be stored together in water-free storage
• These chemicals must be kept away from flammables and alkali metals

Question 9

What should be kept in mind for glassware?

Answer 9

All storage shelves should be well fastened and able to support the weight.
Glassware should be unmarred

Question 10

How to check electrical safety:

Answer 10

make sure power cords aren’t exposed, are in a neat manner, are not in contact with water, don’t clutter a space, kept away from hot surfaces, are not overloaded by high current devices (50 watts)

Question 11

Useful Lab instruments:

Answer 11

Hot plates
thermometers (NO MERCURY)
stirring magnets and boiling stones
water, oil, and sand baths
ultra sonic baths (sonicates)
Lyophilizes (freeze dryers)
Centrifuges (low, high, and ultra)
Autoclaves

Question 12

max noise level?

Answer 12

anything above 80 decibles requires ear protection and NO MUSIC
lab should be quiet

Question 13

Types of waves:

Answer 13

Microwaves
Radio waves
Ultraviolet
Infrared
Laser
Strong Magnets

Question 14

Proper Protocols

Answer 14

*Never walk away from open flames
*When in doubt, ask! Emergency? Call!
* Never use instruments outside of their purpose
* Treat all chemicals carefully with correct labeling and storage
*Don’t touch anything you don’t know
* Wear correct PPE neatly
* Always be neat and never work alone!