ch 9 Flashcards
Transcription factors are made in
cytoplasm
how do transcription factors enter the nucleus
nuclear pore complex, nuclear localization/export signals
karyopherins
Nuclear import/export receptors
hormone receptors
hormones can diffuse directly into cell to interact with receptors
when hormone receptors become activated it will
translate into nucleus and increase gene transcription
what controls transcription
cellular compartmentalization, nuclear architecture, DNA-protein interactions, protein protein interactions, chromatin structure
Pre initiation complex is made of
RNA poly II, 5 general transcription factors, mediators
5 general transcription factors for initiation
recognize and unwind
mediator
interacts with transcription factors that bind to enhancers, stimulate stimulates kinase activity of TFIIH, in conjunctions with enhancer
TFFIID
recognizes TATA box, begins recruitment
TFIIB
orients the complex, aligns RNA polymerase and recruits it
TFIIF
highly associates RNA polymerase II to the DNA
TFIIE
recruits TFIIH
TFIIH
acts as helices and a kinase
what activates C-terminal domain and activates the polymerase
phosphorylation of C-terminal domain
transcription initiation steps
promoter melting
abortive initiaiton
promoter escape
pausing
pause release
non core transcription factors
activate or repress transcription
activators
stimulate recruitment/binding of general transcription factors/ RNA pol II, enzymatic activity of general transcription machinery, chromatin remodeling
transcription factors parts
DNA binding domain motif, dimerization domain, transactivation domain
DNA binding domain motif
helical turn helix, zinc finger, basic leucine sipper, basic helix loop helix
transactivation domain is for
protein protein interaction
dimerization domain
yes idk