Ch. 8 Microbial Genetics Flashcards
Genotype
the genetic makeup of an organism
phenotype
expression of the genes
Short tandem repeats (STRs)
repeating sequences of noncoding DNA
Vertical gene transfer
flow of genetic information from one generation to the next
DNA replication
- Topoisomerase and gyrase relax the strands
- Helicase separates the strands
- A replication fork is created
DNA polymerase
adds nucleotides to the growing DNA strand
Energy needs
– Energy for replication is supplied by nucleotides
– Hydrolysis of two phosphate groups on ATP
provides energy
Risbosomal RNA (rRNA)
integral part of ribosomes
Transfer RNA (tRNA)
transports amino acids during protein synthesis
Messenger RNA (mRNA)
carries coded information from DNA to ribosomes
transcription begins when RNA polymerase binds to the
promoter sequence on DNA
transcription stops when it reaches the
terminator sequence on DNA
Codons
groups of three mRNA nucleotides that code for a particular amino acid
61 sense codons
encode the amino acids
degeneracy
each amino acid is coded by sever codons
Start Codon
AUG
Stop codons
UAA, UAG, UGA
tRNA molecules also have an
anticodon that base-pairs with the codon
exons
regions on DNA that code for proteins
introns
regions of DNA that do not code for proteins
small nuclear ribonucleoprotiens (snRNPs)
remove introns and splice exons together
repression
inhibits gene expression and decreases enzyme synthesis
-mediated by repressors, proteins that block transcription
Induction
inhibits gene expression and decreases enzyme synthesis
- initiated by an inducer
Promoter
segment of DNA where RNA polymerase initiates transcription of structural genes
Operator
segment of DNA that controls transcription of structural genes
Operon
set of operator and promoter sites and the structural genes they control
inducible operon
structural genes are not transcribes unless an inducer is present
repressible operons
structural genes are transcribes until they are turned off
corepressor
represses the expression of genes
catabolite repression
inhibits cells from using carbons sources other than glucose
cyclic AMP (cAMP)
builds up in a cell when glucose is not available
riboswitch
part of an mRNA molecule that binds to a substrate and changes the mRNA structure
microRNAs (miRNAs)
base pair with mRNA to make it double-stranded
mutation
a permanent change in the base sequence of DNA
Mutagens
agents that cause mutations
Spontaneous mutations
occur in the absence of a mutagen
base substitution (point mutation)
change in one base in DNA
missense mutation
base substitution results in change in an amino acid
nonsense mutation
base substitution results in a nonsense (stop) codon
frameshift mutations
-insertion or deletion of one or more nucleotide pairs
-shifts the translational “reading frame”
Nitrous acid
causes adenine to bind with cytosine instead of thymine
nucleoside analog
incorporates into DNA in place of a normal base; causes mistakes in base pairing
photolyases
separate thymine dimers
nucleotide excision repair
enzymes cut out incorrect bases and fill in correct bases
Spontaneous mutation rate
1 in 10^9 replicated base pairs or 1 in 10^6 replicated genes
mutagens increase the mutation rate to
per 10^-5 or 10^-3 replicated gene
positive (direct) selection
detects mutant cells because they grow or appear different than unmutated cells
negative (indirect) selection
detects mutant cells that cannot grow or perform a certain function
auxtotroph
mutant that has a nutritional requirement absent in the parent
- use of replica plating
Ames test
exposes mutant bacteria to mutagenic substances to measure the rate of reversal of the mutation
Genetic recombination
exchange of genes between two DNA molecules; creates genetic diversity
Crossing over
two chromosomes break and rejoin, resulting in the insertion of foreign DNA into the chromosomes
Vertical gene transfer
transfer of genes from an organism to its offspring
Horizontal gene transfer
transfer of genes between cells of the same generation
moblie genetic elements
move from one chromosome to another or from one cell to another
plasmids
self-replicating circular pieces of DNA
Conjugative plasmid
carries genes for sez pili and transfer of the plasmids
dissimilation plasmids
encode enzymes for the catabolism of unusual compounds
resistance factors (R factors)
encode antibiotic resistance