Ch. 7

Question 1

Sepsis

Answer 1

refers to bacterial contamination

Question 2

asepsis

Answer 2

the absence of significant contamination
- aseptic surgery techniques prevent the microbial contamination of wounds

Question 3

sterilization

Answer 3

removing and destroying all microbial life

Question 4

Commercial sterilization

Answer 4

killing Clostridium botulinum endospores form canned goods

Question 5

Disinfection

Answer 5

destroying harmful microorganisms

Question 6

Antisepsis

Answer 6

destroying harmful microorganisms from living tissue

Question 7

degerming

Answer 7

the mechanical removal of microbes from a limited area

Question 8

sanitization

Answer 8

lowering microbial counts on eating utensils to safe levels

Question 9

Biocide (germicide)

Answer 9

treatments that kill microbes

Question 10

Bacteriostasis

Answer 10

inhibiting, not killing, microbes

Question 11

Effectiveness of treatment depends on:

Answer 11

– Number of microbes
– Environment (organic matter, temperature,
biofilms)
– Time of exposure
– Microbial characteristics

Question 12

Actions of Microbial Control Agents

Answer 12

• Alteration of membrane permeability
• Damage to proteins (enzymes)
• Damage to nucleic acids
  Actions of Microbial Control Agents
  (2 of 2)

Question 13

Heat

Answer 13

denatures enzymes

Question 14

Thermal death point (TDP)

Answer 14

lowest temp at which all cells in a liquid culture are killed in 10 min

Question 15

Thermal death time (TDT)

Answer 15

minimal time for all bacteria in a liquid culture to be killed at a particular temp.

Question 16

Decimal reduction tiem (DRT)

Answer 16

minutes to kill 90% of a specific population of bacteria at a given temp.